G protein-coupled receptor for diapause hormone, an inducer of Bombyx embryonic diapause
G protein-coupled receptor for diapause hormone, an inducer of Bombyx embryonic diapause
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DOI：
10.1016/j.bbrc.2006.03.085
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发表时间：
2006-05-26
影响因子：
3.1
通讯作者：
Yaginuma, T
中科院分区：
文献类型：
--
作者：
Homma, T;Watanabe, K;Yaginuma, T
滞育激素是第一种被确定为抑制后代发育的母体控制因子的化学物质。然而，中胚层分裂后，激素将信号转导到卵母细胞诱导胚胎滞育的分子机制尚未完全了解。在这里，我们描述了一个G蛋白偶联滞育激素受体的cDNA与7个跨膜结构域。其氨基酸序列与哺乳动物神经介肽U和昆虫调节肽（FXPRL-酰胺C-末端）的受体具有高度相似性。当在非洲爪蟾卵母细胞系统中表达时，与其他的FXPR/KL-酰胺肽相比，该受体对滞育激素表现出最高的亲和力（EC 50，类似于70 nM）。滞育激素没有酰胺化的C-末端，从来没有诱导胚胎滞育在体内，在这个异源表达系统中没有效果。该mRNA在蛹-成虫发育过程中在卵巢中表达。这些结果有力地表明，该cDNA编码滞育激素受体。(c)2006年爱思唯尔公司All rights reserved.
Bombyx diapause hormone was the first chemical substance identified as a maternal control factor that arrests offspring development. However, the molecular mechanisms by which the hormone transduces the signal to the oocyte that induces embryonic diapause immediately after mesoderm segmentation are not fully understood. Here, we describe a cDNA for a G protein-coupled diapause hormone receptor with seven transmembrane domains. Its amino-acid sequence shows a high level of similarity to the receptors of mammalian neuromedin U and insect regulatory peptide, an FXPRL-amide C-terminus. When expressed in a Xenopus oocyte system, the receptor exhibited the highest affinity (EC50, similar to 70 nM) for diapause hormone, when compared with other Bombyx FXPR/KL-amide peptides. Diapause hormone without amidation at the C-terminus, which never induces embryonic diapause in vivo, had no effect in this heterologous expression system. The mRNA is expressed in the ovaries during Bombyx pupal-adult development. These results strongly indicate that the cDNA encodes the diapause hormone receptor. (c) 2006 Elsevier Inc. All rights reserved.