Optimised microcomputer-guided quantitative microradiography on dental mineralised tissue slices.
Optimised microcomputer-guided quantitative microradiography on dental mineralised tissue slices.
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优化的微计算机引导的牙科矿化组织切片定量显微放射成像。
DOI：
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发表时间：
1987
影响因子：
3.5
通讯作者：
J. Noordmans
中科院分区：
文献类型：
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作者：
E. D. J. D. Jong;J. T. Bosch;J. Noordmans
人们充分认识到，接触显微放射照相法是最直接的方法，可以提供钙化组织矿物质含量及其损失的位置相关信息。我们研制了一种微机引导的显微射线照相系统，其特点是实验人员操作速度快，误差小。用锯床切割75微米厚的牙齿组织切片。在胶片（Kodak SO-253）上制作齿片和铝阶梯楔的图像（Cu K α辐射）（曝光10-15秒）。在配备有XY工作台（0.5微米步长）的密度计（1微米X 30微米狭缝）中扫描阶梯楔和齿切片的图像。微机（Apple IIe）编程控制XY工作台并记录光学膜透射。图像的扫描图显示在计算机屏幕上。校准步骤光学膜透射值由操作员通过将计算机生成的条调整到屏幕上显示的各个步骤上来确定。该程序利用阶楔数据，用四次多项式近似计算胶片的剂量-密度关系。由此产生的曲线用于将齿切片数据转换为矿物体积百分比。为了能够计算总矿物损失（kg m-2）（损失在深度上的积分），操作员在显示器上添加假设的完好搪瓷图。这是通过调整计算机生成的酒吧扫描显示在屏幕上的牙齿切片。由显微射线照相系统产生的图像的分辨能力为3微米X 30微米。通过对随机误差的分析，并与齿片化学分析结果进行比较，认为矿物体积百分数的误差为4%。从牙齿切片的显微射线照相图像开始，需要5分钟以获得纸上的显微射线照相曲线并获得矿物质损失值。
It is well realised that the contact microradiographic method is the most direct method that gives position-dependent information on the mineral content of calcified tissue and its loss. We developed a microcomputer-guided microradiographic system which features fast operation by the experimenter with a low appearance of errors. Tooth tissue slices of 75 micron thickness are cut with a sawing machine. Images (Cu K alpha radiation) of the tooth slice and an aluminium step wedge (exposure 10-15 s) are made on film (Kodak SO-253). The images of step wedge and tooth slice are scanned in a densitometer (1 micron X 30 microns slit), which is fitted with an XY table (0.5 micron steps). A microcomputer (Apple IIe) is programmed to control the XY table and to record the optical film transmission. Scans of the images are plotted on the computer screen. The calibration step optical film transmission values are determined by the operator by adjusting a computer-generated bar onto the individual steps shown on the screen. The dose-density relation of the film is approximated by the program by a fourth-degree polynomial using the step-wedge data. The resulting curve is used to convert the tooth-slice data into a mineral volume percentage. To enable the calculation of total mineral loss (in kg m-2) (loss integrated over depth), the operator adds the assumed diagram for sound enamel onto the display. This is done by adjusting computer-generated bars to the scan of the tooth slice shown on the screen. The resolving power in the image made by the microradiographic system is 3 microns X 30 microns. On the basis of the analysis of random errors and a comparison with chemical analysis of tooth slices we claim that the error in mineral volume percentage amounts to 4% of its value. Starting with a microradiographic image of a tooth slice 5 min are required to obtain a microradiographic curve on paper and to obtain a value for mineral loss.