The detection of fosfomycin resistance genes in Enterobacteriaceae from pets and their owners
The detection of fosfomycin resistance genes in Enterobacteriaceae from pets and their owners
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DOI：
10.1016/j.vetmic.2016.07.019
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发表时间：
2016-09-25
影响因子：
3.3
通讯作者：
Liao, Kang
中科院分区：
文献类型：
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作者：
Yao, Hong;Wu, Dongfang;Liao, Kang
本研究的目的是调查从伴侣动物及其主人身上分离的磷霉素耐药菌株的流行情况和分子特征。采用磷霉素选择性培养基、PCR和测序技术，对中国某兽医教学医院宠物和宠物主人共171份标本进行磷霉素耐药表型和基因型筛查。171份样品中有19株对磷霉素耐药。这些耐磷菌素分离株中，fosA3和fosA基因分别阳性16株和3株。在细菌的染色体和质粒中均检测到fosA3基因。除1株fosA3基因阳性外，其余均为CTX-M产生菌，其中近一半（7/16）也携带rmtB基因。携带fosA3基因的质粒很容易通过结合转移到受体大肠杆菌J53上，使其对多种抗菌药物具有耐药性。遗传结构分别为IS26-385bp-fosA3-1810bp-IS26 （n=11）和IS26-385bp-fosA3-588bp-IS26 （n=5）。分子分型表明，来自犬的2株fosa3阳性分离株与来自宠物主人的分离株基因相同。研究结果表明，从宠物及其主人身上分离的肠杆菌科细菌通过水平转移和克隆扩增的方式，发生了由fosa3介导的磷霉素耐药的主动传播。(C) 2016年Elsevier B.V.出版
The aim of this study was to investigate the prevalence of fosfomycin resistance and molecular characteristic of fosfomycin-resistant strains isolated from companion animals and their owners. A total of 171 samples collected from pets and pet owners in a Chinese veterinary teaching hospital were screened for the presence of phenotype and genotype of fosfomycin-resistance by selective media containing fosfomycin and PCR & sequencing. Among 171 samples tested, nineteen isolates were resistant to fosfomycin. Sixteen and three of these fosfomycin-resistant isolates were positive for fosA3 and fosA genes, respectively. The fosA3 gene was detected both in chromosomes and plasmids in bacteria. All of the fosA3 gene-positive isolates except one were CTX-M producers and nearly half (7/16) of them also harbored the rmtB gene. The fosA3 gene-carrying plasmids, which were readily transferrable to recipient E. coli J53 by conjugation, conferred resistance to multiple antimicrobial agents. Genetic structures were IS26-385bp-fosA3-1810bp-IS26 (n=11) and IS26-385bp-fosA3-588bp-IS26 (n=5). Molecular typing indicated that two fosA3-positive isolates from dogs were genetically identical to the isolates from the pet owners. Our results indicated that active transmission of fosA3-mediated fosfomycin resistance has occurred among Enterobacteriaceae isolated from pets and their owners by both horizontal transfer and clonal expansion. (C) 2016 Published by Elsevier B.V.