Novel Point-of-Care Test for Simultaneous Detection of Nontreponemal and Treponemal Antibodies in Patients with Syphilis
Novel Point-of-Care Test for Simultaneous Detection of Nontreponemal and Treponemal Antibodies in Patients with Syphilis
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DOI：
10.1128/jcm.00624-10
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发表时间：
2010-12-01
影响因子：
9.4
通讯作者：
Ballard, Ronald C.
中科院分区：
文献类型：
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作者：
Castro, Arnold R.;Esfandiari, Javan;Ballard, Ronald C.
我们描述了一种即时免疫层析试验，用于同时检测梅毒患者血清中的非密螺旋体抗体和密螺旋体抗体，该试验可作为筛查和确证试验。共1,601份库存血清样本进行了双重测试，并将结果与使用定量快速血浆反应素（RPR）试验和梅毒螺旋体被动颗粒凝集（TP-PA）试验获得的结果进行了比较。与RPR试验相比，当血清RPR滴度≥ 1：2时，双重试验的反应符合率为98.4%，非反应符合率为98.6%。与TP-PA试验相比，密螺旋体线的反应性和非反应性一致性分别为96.5%和95.5%。这些结果表明，双重测试可用于梅毒的血清学诊断在初级卫生保健诊所或资源贫乏的环境，因此提高治疗率，患者可能无法返回他们的实验室结果。
We describe a point-of-care immunochromatographic test for the simultaneous detection of both nontreponemal and treponemal antibodies in the sera of patients with syphilis that acts as both a screening and a confirmatory test. A total of 1,601 banked serum samples were examined by the dual test, and the results were compared to those obtained using a quantitative rapid plasma reagin (RPR) test and the Treponema pallidum passive particle agglutination (TP-PA) assay. Compared to the RPR test, the reactive concordance of the dual test nontreponemal line was 98.4% when the RPR titers of sera were >= 1:2 and the nonreactive concordance was 98.6%. Compared to the TP-PA assay, the reactive and nonreactive concordances of the treponemal line were 96.5% and 95.5%, respectively. These results indicate that the dual test could be used for the serological diagnosis of syphilis in primary health care clinics or resource-poor settings and therefore improve rates of treatment where patients may fail to return for their laboratory results.