Quality of Regenerated Bone Enhanced by Implantation of Octacalcium Phosphate-Collagen Composite
Quality of Regenerated Bone Enhanced by Implantation of Octacalcium Phosphate-Collagen Composite
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DOI：
10.1089/ten.tec.2009.0212
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发表时间：
2010-06-01
影响因子：
3
通讯作者：
Suzuki, Osamu
中科院分区：
文献类型：
--
作者：
Masuda, Taisuke;Kawai, Tadashi;Suzuki, Osamu
本研究的目的是调查是否可以使用结合显微计算机断层扫描（μ CT）分析的机械测试，以评估由颗粒磷酸八钙和胶原（OCP/Col）基质组成的复合材料植入增强的再生骨的质量。以前的研究证实，单独的OCP颗粒或OCP在Col基质中倾向于成熟为骨样羟基磷灰石，并且如果植入各种骨部位，则与其自身的生物降解相结合，促进骨再生。将OCP/Col植入大鼠颅骨临界大小的骨缺损中4至12周，进行显微压痕、力学测试、mCT成像和组织学检查。显微压痕测试显示，再生骨的维氏硬度逐渐增加，在12周内显示出最高值。维氏硬度与mCT和力学性能参数均符合良好;再生骨质量随植入时间的延长而逐渐增加。组织学上，再生骨呈成熟的骨样基质结构，内有骨细胞。再生骨的力学性能与正常对照颅骨基本一致。结果表明，显微压痕测试可用于评价再生骨的质量在初始再生和骨的发展，促进OCP颗粒在Col基质。OCP/Col的植入似乎可以作为确定再生骨质量的模型研究。
The present study was designed to investigate whether mechanical testing in conjunction with microcomputed tomography (mu CT) analysis can be used to evaluate the quality of regenerated bone enhanced by the implantation of a composite composed of granular octacalcium phosphate and collagen (OCP/Col) matrix. Previous studies confirmed that the granules of OCP alone or OCP in Col matrix tend to mature into bone-like hydroxyapatite and enhance bone regeneration coupled with its own biodegradation, if implanted in various bony sites. OCP/Col was implanted in rat calvaria critical-sized bone defect for 4 to 12 weeks for microindentation, mechanical testing, mCT imaging, and histological examinations. The microindentation testing of the regenerated bone revealed a progressive increase of the Vickers hardness showing the highest value in 12 weeks. The Vickers hardness was in good agreement with both the parameters of mCT and the mechanical property; the quality of regenerated bone increased progressively with the implantation periods. The regenerated bone exhibited a mature bone-like matrix structure with osteocytes histologically. The quality of the regenerated bone was compatible to that of normal control calvaria bone regarding the mechanical properties. The results indicate that microindentation testing can be used to evaluate the quality of regenerated bone in the initial regeneration and the development of bone enhanced by OCP granules within Col matrix. It appears that the implantation of OCP/Col could be a model study to determine the quality of the regenerated bone.