Increased Bacterial Invasion and Differential Expression of Tight-Junction Proteins, Growth Factors, and Growth Factor Receptors in Periodontal Lesions
Increased Bacterial Invasion and Differential Expression of Tight-Junction Proteins, Growth Factors, and Growth Factor Receptors in Periodontal Lesions
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DOI：
10.1902/jop.2014.130740
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发表时间：
2014-08-01
影响因子：
4.3
通讯作者：
Choi, Youngnim
中科院分区：
文献类型：
--
作者：
Choi, Yun S.;Kim, Yong C.;Choi, Youngnim
背景：已知许多病原体调节上皮物理屏障，特别是紧密连接(TJ)蛋白，进入宿主细胞和/或组织。生长因子参与了TJ蛋白的调控。本研究旨在探讨TJ蛋白、生长因子及其受体在牙周炎患者牙龈组织中与细菌侵袭的关系。方法：检测牙周炎患者牙周组织中细菌的存在及连接黏附分子(JAM)-A、阻滞素、表皮生长因子(EGF)、角质形成细胞生长因子(KGF)、胰岛素样生长因子-I(IGF-I)、EGF受体、KGF受体的表达。采用原位杂交法和免疫组织化学方法检测10例牙周炎患者牙周组织中IGF-1受体(IGF-1R)的表达。结果：牙周病变牙周组织中的细菌侵袭力明显高于健康牙周组织。虽然JAM-A和occludin在健康和病变部位的水平没有显著差异，但在病变部位经常观察到JAM-A和闭塞的胞浆异常表达。此外，在与疾病相关的上皮细胞中观察到更多表达JAM-A或occludin的白细胞。与正常对照相比，KGF、IGF-I、IGF-1R在牙周病变组织中的表达差异有统计学意义。结论：生长因子和TJ蛋白的异常表达可能是牙周病变细菌侵袭增加和疾病进展的原因之一。
Background: Many pathogens are known to modulate epithelial physical barriers, particularly tight-junction (TJ) proteins, to enter host cells and/or tissues. Growth factors have been implicated in the regulation of TJ proteins. The aim of this study is to determine differences in the levels of TJ proteins, growth factors, and their receptors in relation to bacterial invasion in diseased gingival tissues obtained from patients with periodontitis.Methods: The presence of bacteria and expression of junctional adhesion molecule (JAM)-A, occludin, epidermal growth factor (EGF), keratinocyte growth factor (KGF), insulin-like growth factor-I (IGF-I), EGF receptor, KGF receptor, and IGF-1 receptor (IGF-1R) were evaluated in gingival tissues from healthy (n = 10) and diseased (n = 10) sites in patients with periodontitis by in situ hybridization and immunohistochemistry.Results: The bacterial invasion of gingival tissue was increased in periodontal lesions compared with healthy sites. Although the levels of JAM-A and occludin were not significantly different between the healthy and diseased sites, aberrant cytoplasmic expression of JAM-A and occluding was often observed in the lesions. In addition, more leukocytes expressing JAM-A or occludin were observed within the disease-associated epithelia. Compared with the healthy sites, the differential expression of KGF, IGF-I, and IGF-1R was observed in the periodontal lesions. The levels of TJ proteins showed positive correlations with those of growth factors.Conclusion: The aberrant expression of growth factors and TJ proteins may contribute to increased bacterial invasion and disease progression in periodontal lesions.