ISOLATION AND CHARACTERIZATION OF NUCLEAR POLYHEDROSIS VIRUSES FROM THE BEET ARMYWORM SPODOPTERA-EXIGUA (LEPIDOPTERA, NOCTUIDAE) FOUND IN SHIGA, JAPAN
ISOLATION AND CHARACTERIZATION OF NUCLEAR POLYHEDROSIS VIRUSES FROM THE BEET ARMYWORM SPODOPTERA-EXIGUA (LEPIDOPTERA, NOCTUIDAE) FOUND IN SHIGA, JAPAN
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DOI：
10.1303/aez.29.105
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发表时间：
1994-02-01
影响因子：
1.3
通讯作者：
MAEDA, S
中科院分区：
文献类型：
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作者：
KONDO, A;YAMAMOTO, M;MAEDA, S
在日本滋贺采集了11只表现出核型多角体病毒（NPV）感染症状的甜菜夜蛾幼虫。从每只幼虫中分离的NPV分为两组。第1组菌株产生立方多面体，而第2组菌株产生二十面体多面体。病毒DNA的限制性内切酶图谱在同一组内是相同的。第1组的限制性内切酶谱与苜蓿银纹夜蛾核型多角体病毒（Autographa californica NPV，AcNPV）相似，第2组的限制性内切酶谱与S.在加州分离的甜菜核型多角体病毒。SDS-聚丙烯酰胺凝胶电泳分析的多面体也表明，SeNPVs不同的两组之间。第1组SeNPVs能感染SF-21和TN-368细胞，第2组则不能。此外，第1组SeNPVs还能感染斜纹夜蛾、小菜蛾和粘虫。甜菜幼虫而不是家蚕幼虫。第2组SeNPVs能感染S.甜菜夜蛾幼虫，但不含S.斜纹夜蛾、小菜蛾或B. B.桑幼虫SeNPV#5（组1）和SeNPV#1（组2）的LD_（50）分别为58和4.8个多角体/幼虫。这些结果表明，在滋贺分离的SeNPVs由两个遗传和表型不同的群体组成。
Eleven larvae of the beet armyworm, Spodoptera exigua, showing symptoms of nuclear polyhedrosis virus (NPV) infection were collected in Shiga Prefecture, Japan. NPVs isolated from each larva were divided into two groups. Group 1 isolates produced cuboidal polyhedra, while group 2 isolates produced icosahedral polyhedra. The restriction endonuclease patterns of viral DNA within the same group were identical. The restriction endonuclease patterns of group 1 were similar to those of Autographa californica NPV (AcNPV), whereas the patterns of group 2 were similar to S. exigua NPV isolated in California. SDS-polyacrylamide gel electrophoresis analysis of polyhedra also indicated that SeNPVs differed between the two groups. Group 1 SeNPVs could infect the cell lines SF-21 and TN-368 and group 2 SeNPVs could not. Furthermore, group 1 SeNPVs were able to infect Spodoptera litura, Plutella xylostella, and S. exigua larvae but not Bombyx mori larvae. Group 2 SeNPVs could infect S. exigua larvae, but not S. litura, P. xylostella, or B. mori larvae. The LD50 of SeNPV #5 (group 1) and SeNPV #1 (group 2) were 58 and 4.8 polyhedra per larva, respectively. These results indicated that the SeNPVs isolated in Shiga Prefecture were composed of two genetically and phenotypically distinct groups.