The combined effect of delayed application of yeast biocontrol agents and fruit curing for the inhibition of the postharvest pathogen Botrytis cinerea in kiwifruit
The combined effect of delayed application of yeast biocontrol agents and fruit curing for the inhibition of the postharvest pathogen Botrytis cinerea in kiwifruit
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DOI：
10.1016/s0925-5214(99)00003-4
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发表时间：
1999-07-01
影响因子：
7
通讯作者：
Ganesh, S
中科院分区：
文献类型：
--
作者：
Cook, DWM;Long, PG;Ganesh, S
生物防治剂的应用和利用诱导的宿主抗性来抑制病原体感染常常被分开研究。本研究的重点是应用酵母BCA单独和猕猴桃烘烤和BCA应用之间的相互作用抑制病原体灰葡萄孢的感染。猕猴桃果梗在自然剥离层处被去除，并且果梗伤口被用于评估多达五种酵母候选物的功效，在B之后以增加的延迟间隔施用。灰斑激发。其他水果用水果腌制（在10 ° C下孵育）和局部施用酵母的组合和各种顺序处理。所有酵母候选物在与B同时施用或在B后96小时施用后赋予显著水平的生物防治。灰霉病接种生物防治活性进一步增加与BCA和水果固化相结合的加性效应，但只有当BCA的应用后，96小时的水果固化。这些结果表明，一定程度的保护猕猴桃可以实现与应用酵母的花梗伤口。如果猕猴桃开始腌制，宿主抗性机制可能不是病原体B所特有的。灰霉病，因为用于生物防治的附生微生物的效果似乎类似地被在固化的前24-48小时中诱导的因素降低。(C)1999 Elsevier Science B. V.保留所有权利。
Application of biocontrol agents (BCAs) and use of induced host resistance for the inhibition of pathogen infection have often been examined separately. The present study focused on application of yeast BCAs alone and the interaction between kiwifruit curing and BCA application for inhibition of infection by the pathogen Botrytis cinerea. Kiwifruit pedicels were removed at the natural abscission layer and the pedicel wound was used to evaluate efficacy of up to five yeast candidates, applied at increasing delay intervals after B. cinerea challenge. Other fruit were treated with combinations and various sequences of fruit curing (incubation at 10 degrees C) and topical yeast application. All yeast candidates conferred a significant level of biocontrol following applications made simultaneously with, or up to 96 h after B. cinerea inoculation. Biocontrol activity was further increased with an additive effect of BCA and fruit curing combined but only when BCA application was made after 96 h of fruit curing. These results suggest that a degree of protection of the kiwifruit could be achieved with the application of the yeast to the pedicel wound. If kiwifruit curing is initiated, host resistance mechanisms may not be specific to the pathogen, B. cinerea, since the effect of epiphytic microbes used for biocontrol appear to be similarly reduced by factors induced in the first 24-48 h of curing. (C) 1999 Elsevier Science B.V. All rights reserved.