Effect of sodium butyrate on synthesis of specific proteins by human breast-carcinoma cells.
Effect of sodium butyrate on synthesis of specific proteins by human breast-carcinoma cells.
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DOI：
10.1038/bjc.1980.287
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发表时间：
1980-10
影响因子：
8.8
通讯作者：
Howell, A
中科院分区：
文献类型：
--
作者：
Grieve, R J;Woods, K L;Mann, P R;Smith, S C;Wilson, G D;Howell, A
据观察，丁酸钠可以在多种培养的肿瘤细胞系中降低生长速度、改变特定蛋白质的合成并诱导生化和形态分化（Prasad & Sinha，1976）。它对人类细胞系的影响包括：（1）诱导白血病细胞中的红系分化（Andersson等，1979）；（2）诱导神经母细胞瘤细胞中的神经突形成（Prasad＆Kumar，1974）；（3）刺激HeLa细胞（源自宫颈癌）合成糖蛋白激素FSH和hCG及其共同的a亚基（Ghosh＆Cox， 1976 年、1977 年；利布里希等人，1977 年）； (4) 支气管癌系刺激o-亚基的合成(Chou et al., 1977)。我们在此报告，丁酸钠对人乳腺癌细胞中的蛋白质合成具有显着影响。这些作用不限于诱导分化。 o-亚基的产生通常仅限于胎盘组织和某些内分泌细胞。然而，在多种肿瘤类型中已注意到糖蛋白激素及其亚基的不适当合成（Rosen 等，1975）。为了阐明丁酸盐的作用，我们研究了它对 MCF7 人乳腺癌细胞系的影响，涉及 (a) 乳蛋白乳糖的合成。
SODIUM BUTYRATE has been observed to reduce growth rate, alter synthesis of specific proteins and induce biochemical and morphological differentiation, in a variety of cultured tumour-cell lines (Prasad & Sinha, 1976). Its effects on cell lines of human origin include:(1) induction of erythroid differentiation in leukaemia cells (Andersson et al., 1979);(2) induction of neurite formation in neuro-blastoma cells (Prasad & Kumar, 1974);(3) stimulation of synthesis ofthe glyco-protein hormones FSH and hCG, and of their common a-subunit by HeLa cells (derived from cervical carcinoma)(Ghosh & Cox, 1976, 1977; Lieblich et al., 1977); and (4) stimulation of synthesis of o-subunit by a bronchial-carcinoma line (Chou et al., 1977). We report here that sodium butyrate has marked effects on protein synthesis in human breast-carcinoma cells. These actions are not limited to induction of differentiation. Production of o-subunit is normally confined to placental tissue and to certain endocrine cells. However, inappropriate synthesis of glycoprotein hormonesand their subunits has been noted in a variety of tumour types (Rosen et al., 1975). In order to clarify the actions of butyrate we have studied its effects on the MCF7 human breast-carcinoma line, with regard to synthesis of (a) the milk protein lactal-