Studies on gastric digestion of protein and carbohydrate, gastric secretion and exocrine pancreatic secretion in the growing pig
Studies on gastric digestion of protein and carbohydrate, gastric secretion and exocrine pancreatic secretion in the growing pig
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生长猪胃对蛋白质和碳水化合物的消化、胃液分泌及胰腺外分泌的研究
DOI：
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发表时间：
1983
影响因子：
3.6
通讯作者：
H. Żebrowska
中科院分区：
文献类型：
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作者：
T. Żebrowska;A. Low;H. Żebrowska
1. 六头猪，最初平均活重为 35 公斤，每只猪都安装有凹入插管。它形成于十二指肠短袋的两侧，胰管在十二指肠中开口，并且包含与折返套管中心相连的简单套管。 2.每头猪接受两种日粮：日粮A以小麦淀粉、蔗糖和酪蛋白为主，日粮B以大麦和豆粕为主。每隔 12 小时等量喂食一次。 3.在每个日粮的每头猪的三个12小时期间连续收集消化物和胰液。 4.平均十二指肠输出量：饮食A和B的饮食摄入量分别为：食糜1·80、2·86；干物质1·05、1·03；氮气 1·05、1·06；溶于三氯乙酸 (TCA) N 7·69、9·10；葡萄糖0·97、0·89。对于饮食 A，TCA 可溶性氮占总氮的比例在 12 小时内从 13% 上升至 50%，而饮食 B 在 12 小时内约为 50%。 5. 平均总胃蛋白酶 (EC 3.4.23.1) 活性（单位/24 小时）分别为 760449（饮食 A）和 1466571（饮食 B）。 6. 计算得出，饮食 A 和 B 的唾液和胃分泌量分别约为 4 公斤/24 小时和 8 公斤/24 小时。 7. 饮食 A 和 B 的胰液平均流量（克/24 小时）分别为： 胰液 1204、2182；蛋白质10·94、12·10； N 1·98, 2·14;灰分 9·46、17·31；钠 3·88、6·91；钾0·23、0·54：钙0·031、0·046；磷0·024、0·026。 8. 日粮 A 和 B 的平均总酶活性（单位 × 10−3/24 h）分别为： 胰蛋白酶 (EC 3.4.21.4) 138、114；胰凝乳蛋白酶 (EC 3.4.21.1) 84, 84;羧肽酶 A (EC 3.4.2.1) 5, 4;羧肽酶 B (EC 3.4.2.2) 15, 17;淀粉酶(EC 3.2.1.1) 1061, 981。 9.经计算，24小时内唾液和胃分泌物中内源N的最低量为0·3～0·6 g。这假设十二指肠插管之前没有发生氮吸收。
1. Six pigs, initially of 35 kg mean live weight, were each fitted with a re-entrant cannula. This was formed on either side of a short pouch of duodenum into which the pancreatic duct opened and which contained a simple cannula linked to the centre of the re-entrant cannula. 2. Each pig received two diets: diet A was based on wheat starch, sucrose and casein, while diet B was based on barley and soya-bean meal. The diets were given in equal amounts at 12 h intervals. 3. Digesta and pancreatic juice were collected continuously during three 12 h periods for each pig on each diet. 4. Mean duodenal output: dietary intake values for diets A and B respectively were: digesta 1·80, 2·86; dry matter 1·05, 1·03; nitrogen 1·05, 1·06; trichloroacetic acid (TCA)-soluble N 7·69, 9·10; glucose 0·97, 0·89. For diet A the proportion of TCA-soluble N in total N rose from 13 to 50% during 12 h, while it was approximately 50% throughout 12 h for diet B. 5. Mean total pepsin (EC 3.4.23.1) activities (units/24 h) were 760449 (diet A) and 1466571 (diet B). 6. Salivary and gastric secretions were calculated to be approximately 4 and 8 kg/24 h for diets A and B respectively. 7. Mean flows in pancreatic juice (g/24 h) for diets A and B respectively were: juice 1204, 2182; protein 10·94, 12·10; N 1·98, 2·14; ash 9·46, 17·31; sodium 3·88, 6·91; potassium 0·23, 0·54: calcium 0·031, 0·046; phosphorus 0·024, 0·026. 8. Mean total enzyme activities (units × 10−3/24 h) for diets A and B respectively were: trypsin (EC 3.4.21.4) 138, 114; chymotrypsin (EC 3.4.21.1) 84, 84; carboxypeptidase A (EC 3.4.2.1) 5, 4; carboxypeptidase B (EC 3.4.2.2) 15, 17; amylase (EC 3.2.1.1) 1061, 981. 9. It was calculated that the minimum amount of endogenous N from saliva and gastric secretion was 0·3–0·6 g in 24 h. This assumes no absorption of N occurred anterior to the duodenal cannula.
无蛋白饮食对甲状腺功能正常和甲状腺功能亢进大鼠肌肉蛋白周转和氮保存的影响。
DOI：
10.1042/bj2170471
发表时间：
1984
期刊：
The Biochemical journal
影响因子：
--
作者：
Carter,WJ;vanderWeijdenBenjamin,WS;Faas,FH
通讯作者：
Faas,FH
低蛋白高碳水化合物饮食会引起大鼠血清甲状腺氨酸结合蛋白的变化。
影响因子：
4.8
作者：
Young,RA;Braverman,LE;Rajatanavin,R
通讯作者：
Rajatanavin,R