PrP protein is associated with follicular dendritic cells of spleens and lymph nodes in uninfected and scrapie‐infected mice
PrP protein is associated with follicular dendritic cells of spleens and lymph nodes in uninfected and scrapie‐infected mice
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PrP 蛋白与未感染和痒病感染小鼠的脾脏和淋巴结滤泡树突状细胞相关
DOI：
10.1002/path.1711680412
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
M. Bruce
中科院分区：
文献类型：
--
作者：
P. McBride;P. Eikelenboom;G. Kraal;H. Fraser;M. Bruce
宿主蛋白PrP的异常形式在感染瘙痒病的动物的中枢神经系统中积聚。在此，免疫细胞化学方法在未感染的小鼠的脾、淋巴结、Peyer‘s结、胸腺和胰腺的组织切片中检测到PrP蛋白，并从感染了一系列鼠传代瘙痒病菌株和牛海绵状脑病(BSE)的小鼠中检测到PrP蛋白。在脾、淋巴结和Peyer‘s斑中，PrP阳性细胞的位置、形态和免疫复合物捕获功能被鉴定为滤泡树突状细胞(FDC)，而在胸腺中，PrP阳性细胞似乎是两种基质细胞：交错突细胞(IDC)和皮质上皮细胞。在胰腺中，含PrP的细胞局限于朗格汉斯的胰岛。虽然PrP免疫球蛋白在瘙痒病感染和未感染小鼠的组织中分布相同，但有证据表明PrP在感染小鼠的FDC中以异常形式聚集。如果PrP对病原体复制是必不可少的，我们的结果表明FDC是瘙痒病和疯牛病在脾和淋巴结复制的场所。
Abnormal forms of a host protein, PrP, accumulate in the central nervous system in scrapie‐affected animals. Here, PrP protein was detected immunocytochemically in tissue sections of spleen, lymph node, Peyer's patches, thymus, and pancreas from uninfected mice and from mice infected with a range of mouse‐passaged scrapie strains and bovine spongiform encephalopathy (BSE). In the spleen, lymph node and Peyer's patches, PrP‐positive cells were identified as follicular dendritic cells (FDC) by their location, appearance, and immune complex trapping function, whereas in the thymus they appeared to be two types of stromal cells: interdigitating cells (IDC) and cortical epithelial cells. In pancreas, PrP‐containing cells were confined to the islets of Langerhans. Although the distribution of PrP immunola belling was the same in tissues from scrapie‐affected and uninfected mice, there was evidence that PrP accumulated in abnormal forms in FDC of infected mice. If, as is likely, PrP is essential for agent replication, our results suggest that FDC are the site of scrapie and BSE replication in the spleen and lymph node.
使用非纯化组织制剂中的抗 SAF 抗体检测痒病相关原纤维 (SAF) 蛋白。
DOI：
10.1099/0022-1317-67-4-671
发表时间：
1986
期刊：
The Journal of general virology
影响因子：
--
作者：
Rubenstein,R;Kascsak,RJ;Merz,PA;Papini,MC;Carp,RI;Robakis,NK;Wisniewski,HM
通讯作者：
Wisniewski,HM