Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells
Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells
复制标题
烟曲霉感染人角膜上皮细胞期间甘露糖结合凝集素2的早期表达
DOI：
10.3980/j.issn.2222-3959.2015.01.06
复制
发表时间：
2015-02-18
影响因子：
1.4
通讯作者：
Zhao, Gui-Qiu
中科院分区：
文献类型：
--
作者：
Che, Cheng-Ye;Zhang, Jing-Fen;Zhao, Gui-Qiu
目的：探讨甘露糖结合凝集素2(MBL2)在烟曲霉(AF)感染人角膜上皮细胞(HCECs)中的早期表达。方法：体外培养人角膜上皮细胞，分别于0、0.5、1、2、4、6、8h取材。半定量逆转录聚合酶链式反应(RT-PCR)检测MBL2基因的表达。酶联免疫吸附试验检测培养上清液中MBL2蛋白的表达。结果：正常人脐静脉内皮细胞表达MBL2基因和蛋白。经AF抗原刺激后，培养上清液中MBL2mRNA和蛋白的表达开始增加。MBL2基因的表达高峰在2小时左右。培养0.5h后，上清液中MBL2蛋白逐渐减少。结论：体外培养的人脐静脉内皮细胞有MBL2受体的表达。AF抗原的刺激可使其早期表达增加。
AIM: To evaluate the early expression of mannose binding lectin 2 (MBL2) in human corneal epithelial cells (HCECs) infected by Aspergillus fumigatus (AF).METHODS: HCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h. The expression of MBL2 mRNA was evaluated by semiquantitative reverse transcription -polymerase chain reaction (RT - PCR). The expression of MBL2 protein in supernatant fluid was shown by enzyme linked immunosorbent assay (ELISA). MBL2 protein in HCECs was detected by immunocytochemistry at 0 and 24h.RESULTS: MBL2 mRNA and protein are expressed in normal HCECs. The expression of MBL2 mRNA and protein in supernatant fluid begin to increase after being stimulated with AF antigens. The most significantly peak of MBL2 mRNA is in 2h. The protein of MBL2 in supernatant fluid decrease gradually after 0.5h. The protein in HCECs expression increase after stimulation of 24h.CONCLUSION: MBL2 receptor expressed in normal HCECs in vitro. The stimulation by AF antigens can increase the early expression of it.