PAH-degradation by Paenibacillus spp. and description of Paenibacillus naphthalenovorans sp nov., a naphthalene-degrading bacterium from the rhizosphere of salt marsh plants
PAH-degradation by Paenibacillus spp. and description of Paenibacillus naphthalenovorans sp nov., a naphthalene-degrading bacterium from the rhizosphere of salt marsh plants
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DOI：
10.1099/00207713-52-1-131
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发表时间：
2002-01-01
影响因子：
2.8
通讯作者：
Häggblom, MM
中科院分区：
文献类型：
--
作者：
Daane, LL;Harjono, I;Häggblom, MM
以萘或菲为唯一碳源，从石油污染的底泥和盐沼根际中分离到拟青霉属细菌，并用表型、形态和分子生物学技术对其进行了鉴定。根据菌落形态和多环芳烃降解模式对菌株进行了分组。菲降解菌在固体培养上产生斑驳菌落，经脂肪酸甲酯和16S rRNA基因序列分析鉴定为巴氏假单胞菌。相反，根据脂肪酸甲酯和16S rRNA基因序列分析，具有粘液状菌落形态的降解萘的菌株与巴氏杆菌有较远的亲缘关系。粘液分离株的主要脂肪酸组成为15：0 Anteiso、16：1 omega11c、16：0和17：0 Anteiso，平均分别为50.5%、12.0%、11.2%和6.5%。其DNA中G+C含量在47~52mol%之间。16S rDNA序列分析表明，该菌株与巴氏拟青霉的相似性最高(小于或等于94%)。此外，基于16S rDNA序列的系统发育分析表明，粘液分离株在拟青霉属内形成了一个明显的聚类群。DNA-DNA杂交实验表明，该模式菌株与粘液型菌株PR-N1之间的DNA相似性仅为6%。根据形态、表型和分子生物学特征，该菌株可归类为一种新的拟青霉属(Paenibacillusnapenienovorans sp.)11月提出以PR-N1(T)(=ATCC BAA-206(T)=DSM 14203(T))为模式菌株。
Bacteria belonging to the genus Paenibacillus were isolated by enrichment from petroleum-hydrocarbon-contaminated sediment and salt marsh rhizosphere using either naphthalene or phenanthrene as the sole carbon source, and were characterized using phenotypic, morphological and molecular techniques. The isolates were grouped by their colony morphologies and polyaromatic hydrocarbon- degradation patterns. Phenanthrene-degrading isolates produced mottled colonies on solid media and were identified as P. validus by fatty acid methyl ester and 16S rRNA gene sequence analyses. In contrast, the naphthalene-degrading isolates with mucoid colony morphology were distantly related to Paenibacillus validus, according to fatty acid methyl ester and 16S rRNA gene sequence analyses. The predominant fatty acids of the mucoid isolates were 15:0 anteiso, 16:1omega11c, 16:0 and 17:0 anteiso, constituting, on average, 50.5, 12.0, 11.2 and 6.5% of the total, respectively. The G+C contents of their DNA ranged from 47 to 52 mol%. The 16S rDNA sequence analysis revealed the highest (less than or equal to94%) similarity to P. validus. In addition, phylogenetic analyses based on 16S rDNA sequences showed that the mucoid isolates formed a distinct cluster within Paenibacillus. DNA-DNA hybridization experiments showed only a 6% DNA similarity between the type strain of P. validus and mucoid strain PR-N1. On the basis of the morphological, phenotypic and molecular data, the naphthalene-degrading isolates merit classification as a new Paenibacillus species, for which the name Paenibacillus naphthalenovorans sp. nov. is proposed, with PR-N1(T) (= ATCC BAA-206(T) = DSM 14203(T)) as the type strain.