Development of novel murine mammary imaging windows to examine wound healing effects on leukocyte trafficking in mammary tumors with intravital imaging.
Development of novel murine mammary imaging windows to examine wound healing effects on leukocyte trafficking in mammary tumors with intravital imaging.
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DOI：
10.1080/21659087.2015.1125562
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Richmond, Ann
中科院分区：
文献类型：
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作者：
Sobolik, Tammy;Su, Ying-Jun;Richmond, Ann
我们开发了乳腺成像窗口（MIWs），以评估白细胞浸润和癌细胞扩散的小鼠乳腺肿瘤共聚焦显微镜成像。以前的技术依赖于手术切除皮瓣来成像肿瘤微环境，将成像时间限制在几个小时。乳腺成像窗口的利用提供了肿瘤微环境的活体成像的扩展。我们的特点优势，并确定了一些以前未描述的MIW技术的潜在弱点。通过迭代增强经皮入口，我们定义了提高质量和延长共聚焦成像时间的条件，用于成像肿瘤微环境中的关键细胞-细胞相互作用。
We developed mammary imaging windows (MIWs) to evaluate leukocyte infiltration and cancer cell dissemination in mouse mammary tumors imaged by confocal microscopy. Previous techniques relied on surgical resection of a skin flap to image the tumor microenvironment restricting imaging time to a few hours. Utilization of mammary imaging windows offers extension of intravital imaging of the tumor microenvironment. We have characterized strengths and identified some previously undescribed potential weaknesses of MIW techniques. Through iterative enhancements of a transdermal portal we defined conditions for improved quality and extended confocal imaging time for imaging key cell-cell interactions in the tumor microenvironment.