CHROMATIN CONFIGURATIONS AND MEIOTIC COMPETENCE OF OOCYTES ARE RELATED TO FOLLICULAR DIAMETER IN NONSTIMULATED RHESUS-MONKEYS
CHROMATIN CONFIGURATIONS AND MEIOTIC COMPETENCE OF OOCYTES ARE RELATED TO FOLLICULAR DIAMETER IN NONSTIMULATED RHESUS-MONKEYS
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DOI：
10.1095/biolreprod48.2.349
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发表时间：
1993-02-01
影响因子：
3.6
通讯作者：
BAVISTER, BD
中科院分区：
文献类型：
--
作者：
SCHRAMM, RD;TENNIER, MT;BAVISTER, BD
本研究的具体目的是比较非促性腺激素刺激的恒河猴完整窦状卵泡的大小和成熟囊泡（GV）卵母细胞的减数分裂能力、直径和染色质构型之间的关系。从九只处于月经周期卵泡期的正常周期猴和两只无周期猴的切除卵巢中解剖出完整的窦状卵泡。根据直径将卵泡分类：I（200-450 μ m）、II（451-700 μ m）、III（701-1000 μ m）和IV（> 1000 μ m）。从卵泡中释放卵丘封闭的卵母细胞，测量（直径）并立即固定或在含有0.5 μ g/ml绵羊FSH、10 μ g/ml绵羊LH和10%小牛血清的改良CMRL培养基中培养48小时。Hoechst染色后，根据与核仁周边的关联程度（包裹或“镶边”），在GV卵母细胞中鉴定出三种不同的染色质组织模式（GV 1 -3）。在直径> 450 μ m的有腔卵泡中，培养前卵母细胞的核仁周围包裹（GV 3）和培养后卵母细胞的减数分裂成熟（中期II）随着有腔卵泡的生长以分级的方式增加（p < 0.01）。虽然56.3%的卵母细胞从大（> 1000 μ m）卵泡完成成熟，少数（9.3%）卵母细胞从小（200-450 μ m）卵泡有能力在体外成熟。在培养0 h时，IV类卵泡比I、II和III类卵泡含有更多（p < 0.01）GV 3卵母细胞和更少（p < 0.01）GV 1卵母细胞。培养48 h后，GV 3卵母细胞的百分比下降（p < 0.05），中期II卵母细胞相应增加，GV 1和GV 2卵母细胞在48 h后没有进入GV状态。在未受刺激的恒河猴中，来自有腔卵泡的卵母细胞并非都具有相同的成熟能力，但随着卵泡的生长，卵母细胞经历核仁包裹并获得减数分裂能力。
Specific aims of this study were to compare relationships between size of intact antral follicles and meiotic competence, diameters, and chromatin configurations of germinal vesicle (GV) oocytes in non-gonadotropin-stimulated rhesus monkeys. Intact antral follicles were dissected from excised ovaries of nine normally cycling monkeys in the follicular phase of the menstrual cycle and of two acyclic monkeys. Follicles were classified according to diameter: I (200-450 mum), II (451-700 mum), III (701-1000 mum) and IV (> 1000 mum). Cumulus-enclosed oocytes were released from follicles and either measured (diameter) and fixed immediately or cultured for 48 h in modified CMRL medium containing 0.5 mug/ml ovine FSH, 10 mug/ml ovine LH, and 10% bovine calf serum. Following Hoechst staining, three distinct patterns of chromatin organization (GV1-3) were identified in GV oocytes according to the degree of association with the nucleolar periphery (encapsulation or ''rimming''). In antral follicles > 450 mum in diameter, perinucleolar encapsulation (GV3) of oocytes before culture and meiotic maturation (metaphase II) of oocytes after culture increased (p < 0.01) with antral follicle growth in a graded fashion. While 56.3% of oocytes from large (> 1000 mum) follicles completed maturation, few (9.3% ) oocytes from small (200-450 mum) follicles were competent to mature in vitro. At 0 h of culture, class IV follicles contained a greater (p < 0.01) proportion of GV3 oocytes and a smaller (p < 0.01) proportion of GV1 oocytes than classes I, II, and III follicles. While the percentage of GV3 oocytes decreased (p < 0.05) by 48 h of culture, with a corresponding increase in metaphase II oocytes, GV1 and GV2 oocytes did not progress in GV status by 48 h. In the nonstimulated rhesus monkey, oocytes from antral follicles are not all equally competent to mature, but undergo nucleolar encapsulation and acquire meiotic competence with follicular growth.