AFLP analysis of genetic relationships in the genus Fosterella L.B. Smith (Pitcairnioideae, Bromeliaceae).
AFLP analysis of genetic relationships in the genus Fosterella L.B. Smith (Pitcairnioideae, Bromeliaceae).
复制标题
Fosterella L.B. 属遗传关系的 AFLP 分析
作者：
M. Rex;Kerstin Patzolt;K. Schulte;G. Zizka;R. Vásquez;P. Ibisch;K. Weising
新热带属Fosterella L.B. Smith（Pitcairnioideae，Bromeliaceae）约有30种，分布于玻利维亚安第斯山脉半干旱至湿润的山地。由于缺乏可供鉴别的性状，种的形态分化比较困难，而且对属内的亲缘关系也知之甚少。在这里，我们提出了77 Fosterella标本的扩增片段长度多态性（AFLP）分析的结果，涵盖18个公认的物种和9个尚未描述的形态种。8个引物组合产生310条带，其被评分为存在/不存在特征。相邻连接树重建显示12个集群（A-L）与不同程度的支持。支持的物种组也恢复了主坐标分析。除少数例外，分子数据证实了形态学定义的物种边界。然而，由于内部分支长度较短，物种组之间的系统发育关系仍然不明确。的AFLP数据进行了补充调查的叶解剖的19种Fosterella。物种的概念和组合进行了讨论的背景下，分子，形态学，解剖学，生态学，和生物学数据。这些数据表明，意外的长距离扩散和创始人事件一直是重要的Fosterella物种。
The neotropical genus Fosterella L.B. Smith (Pitcairnioideae, Bromeliaceae) comprises about 30 species, with a centre of diversity in semiarid to humid habitats of the Andean slopes and valleys of Bolivia. Morphologic differentiation of species is difficult because of a paucity of diagnostic characters, and little is known about the infrageneric phylogeny. Here, we present the results of an amplified fragment length polymorphism (AFLP) analysis of 77 Fosterella specimens, covering 18 recognized species and 9 as-yet undescribed morphospecies. Eight primer combinations produced 310 bands, which were scored as presence/absence characters. Neighbour-joining tree reconstruction revealed 12 clusters (A-L) with various levels of support. Well-supported species groups were also recovered by a principal coordinates analysis. With few exceptions, morphologically defined species boundaries were confirmed by the molecular data. Phylogenetic relationships between species groups remained ambiguous, however, because of short internal branch lengths. The AFLP data were complemented by a survey of the leaf anatomy of 19 Fosterella species. Species concepts and assemblages are discussed in the context of molecular, morphologic, anatomic, ecologic, and biogeographic data. The data suggest that accidental long-distance dispersal and founder events have been important for Fosterella speciation.