Factors influencing the differential sorption of odorant molecules across the olfactory mucosa.
Factors influencing the differential sorption of odorant molecules across the olfactory mucosa.
复制标题
影响气味分子在嗅觉粘膜上的差异吸附的因素。
DOI：
10.1085/jgp.69.3.343
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发表时间：
1977-03
期刊：
影响因子：
--
通讯作者：
Mozell MM
中科院分区：
文献类型：
--
作者：
Hornung DE;Mozell MM
通过使用流动稀释嗅觉仪，氚标记的气味剂通过外鼻孔到达牛蛙完整的嗅囊。刺激后，将动物冷冻在液氮中。然后去除嗅囊的背表面和隆起，并锯成垂直于粘膜表面长轴的切片。将每个切片溶解在组织增溶剂中，并在液体闪烁系统中计数。每个部分的放射性量被用来估计它吸附的气味分子的数量。对于氚化丁醇，从含有外鼻孔的部分到悬于内鼻孔的部分，放射性显著降低。梯度的陡度不受相当大范围的刺激流速、体积和分压的影响。只有当这些参数被推到极端的物理极限时，这种梯度才会发生显著变化。当刺激通过内鼻孔而不是外鼻孔呈现时，丁醇梯度反转其方向，从内到外逐渐减小。与丁醇不同，通过外鼻孔呈现的氚化辛烷相当均匀地分布在粘膜切片中。也就是说，辛烷在整个粘膜中没有显示出分布梯度。这些结果补充了以前的电生理数据，表明一个“色谱样”的差异吸附的气味分子在整个粘膜。
By use of a flow dilution olfactometer, tritium-labeled odorants were presented through the external naris to the bullfrog's intact olfactory sac. After stimulation the animal was frozen in liquid nitrogen. The dorsal surface and eminentia of the olfactory sac were then removed and sawed into sections perpendicular to the long axis of the mucosal surface. Each section was dissolved in a tissue solubilizer and counted in a liquid scintillation system. The amount of radioactivity in each section was used to estimate the number of odorant molecules it sorbed. For tritiated butanol there was a significant decrease in radioactivity from the section containing the external naris to that overhanging the internal naris. The steepness of the gradient was unaffected by a rather large range of stimulus flow rates, volumes, and partial pressures. Only when these parameters were pushed to extreme physical limits did this gradient change significantly. When the stimulus was presented through the internal rather than the external naris, the butanol gradient reversed its direction, decreasing from the internal to external. Unlike butanol, tritiated octane presented through the external naris was rather evenly distributed among the mucosal sections. That is, octane showed no distribution gradient across the mucosa. These results complement previous electrophysiological data that suggested a "chromatographic-like" differential sorption of odorant molecules across the mucosa.