Morphological analysis of the sheathed flagellum of Brucella melitensis.
Morphological analysis of the sheathed flagellum of Brucella melitensis.
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DOI：
10.1186/1756-0500-3-333
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发表时间：
2010-12-09
影响因子：
1.8
通讯作者：
Letesson JJ
中科院分区：
文献类型：
--
作者：
Ferooz J;Letesson JJ
最近的研究表明，B.羊种是有鞭毛的。然而，鞭毛结构仍然很少描述。我们分析了B的极鞘鞭毛的结构。通过透射电镜分析，证明Ryu染色法是一种在光学显微镜下快速观察鞭毛的好方法。透射电镜分析表明，外膜的延伸包围着一个由棒状结构结束的细丝。ΔftcR、ΔfliF、ΔflgE和ΔfliC鞭毛突变体仍然产生空鞘。结果表明，B. Melitensis具有鞘状鞭毛的特征。我们的研究结果还表明，鞭毛鞘的生产是没有直接联系到鞭毛结构组装，不受FtcR主调节。
It was recently shown that B. melitensis is flagellated. However, the flagellar structure remains poorly described. We analyzed the structure of the polar sheathed flagellum of B. melitensis by TEM analysis and demonstrated that the Ryu staining is a good method to quickly visualize the flagellum by optical microscopy. The TEM analysis demonstrated that an extension of the outer membrane surrounds a filament ending by a club-like structure. The ΔftcR, ΔfliF, ΔflgE and ΔfliC flagellar mutants still produce an empty sheath. Our results demonstrate that the flagellum of B. melitensis has the characteristics of the sheathed flagella. Our results also suggest that the flagellar sheath production is not directly linked to the flagellar structure assembly and is not regulated by the FtcR master regulator.