G-protein gamma 7 subunit is selectively expressed in medium-sized neurons and dendrites of the rat neostriatum.
G-protein gamma 7 subunit is selectively expressed in medium-sized neurons and dendrites of the rat neostriatum.
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G 蛋白 γ 7 亚基在大鼠新纹状体的中等大小神经元和树突中选择性表达。
DOI：
10.1002/jnr.490390113
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发表时间：
1994
影响因子：
4.2
通讯作者：
Sutcliffe,JG
中科院分区：
文献类型：
--
作者：
Watson,JB;Coulter2nd,PM;Margulies,JE;deLecea,L;Danielson,PE;Erlander,MG;Sutcliffe,JG
我们用消减杂交技术分离了γ7的克隆，它是一个68个残基的G蛋白γ亚基。Northern印迹和原位杂交显示，γ7亚单位基因主要在新纹状体、伏隔核和嗅结节的中等大小神经元中表达，在海马结构的齿状回和新皮质的II-III、V层有少量表达。γ7mRNA被转移到新纹状体和海马齿状回的神经元树突中。γ7在外周组织中的表达浓度相对较低。γ7在大脑中的选择性表达模式与纹状体丰富的腺酰环化酶ACST、多巴胺受体和高尔夫的α亚单位高度相似，这表明在纹状体中，γ7可能是含有高尔夫α的G蛋白的一个亚单位，它选择性地将多巴胺受体偶联到ACST上。
We used subtractive hybridization to isolate clones of γ7, a 68 residue G‐protein γ subunit. Northern blotting and in situ hybridization reveal that the γ7 subunit mRNA is expressed primarily in medium‐sized neurons of the neostriatum and nucleus accumbens and neurons of the olfactory tubercle, and at low levels in the dentate gyrus of the hippocampal formation and laminae II–III, and V of the neocortex. The γ7 mRNA is translocated into dendrites of neurons in the neostriatum and the dentate gyrus of the hippocampus. γ7 is expressed at relatively very low concentrations in peripheral tissues. The selective pattern of γ7 expression within the brain is highly reminiscent of those of the striatum‐enriched adenylyl cyclase ACST, dopamine receptors, and the α subunit of Golf, suggesting that, in striatum, γ7 may be a subunit of a Golfα‐containing G protein that couples dopamine receptors selectively to ACST.Copyright © 1994 Wiley‐Liss, Inc.