Molecular phylogeny of Laetiporus and other brown rot polypore genera in North America
Molecular phylogeny of Laetiporus and other brown rot polypore genera in North America
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DOI：
10.3852/07-124r2
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发表时间：
2008-05-01
期刊：
影响因子：
2.8
通讯作者：
Banik, Mark T.
中科院分区：
文献类型：
--
作者：
Lindner, Daniel L.;Banik, Mark T.
使用 ITS、核大亚基和线粒体小亚基 rDNA 序列研究了北美 Laetiporus、Leptoporus、Phaeolus、Pycnoporellus 和 Wolfiporia 物种之间的系统发育关系。这些属的成员具有孔状子实膜托、简单的隔膜菌丝，并在多种基质中引起褐腐病。分析表明，Laetiporus 和 Wolfiporia 不是单系的。所有北美 Laetiporus 物种都形成了一个得到良好支持的单系群（“核心 Laetiporus 分支”或 Laetiporus s.s.），但 L. persicinus 除外，该属对有序列数据的属几乎没有亲和力。根据 GenBank 的数据，南半球物种 L. portentosus 也远远落在核心 Laetiporus 进化枝之外。发现扩张菌丝 (Wolfiporia dilatohypha) 是核心 Laetiporus 分支的姐妹群。 Phaeolus、Pycnoporellus 和核心 Laetiporus 分支的成员均属于多孔菌的 Antrodia 分支，而 Leptoporus mollis 和 Laetiporus portentosus 则属于多孔菌的静脉类分支。与之前的研究相反，Wolfiporia cocos 分离株也属于牛樟芝分支。将 W cocos 置于核心多孔状进化枝中。 ITS 分析解析了 Laetiporus s.s. 内的八个进化枝，其中三个可能代表未描述的物种。使用三个 DNA 区域的组合分析解析了 Laetiporus s.s. 内的五个主要进化枝：含有针叶树栖息物种的进化枝（“Conifericola clade”）、含有 L. cincinnatus 的进化枝（“Cincinnatus clade”）、含有 L. sulfureus s.s. 的进化枝。具有黄色孔的分离株（“Sulphureus clade I”），含有 L. sulfureus s.s. 的分支。具有白色孔的分离株（“Sulphureus clade II”）和含有 L. gilbertsonii 的分支以及来自加勒比地区的不明分离株（“Gilbertsonii clade”）。虽然有强大的支持。对于 Laetiporus 分支核心内的群体而言，这些群体之间的关系仍未得到很好的解决。
Phylogenetic relationships were investigated among North American species of Laetiporus, Leptoporus, Phaeolus, Pycnoporellus and Wolfiporia using ITS, nuclear large subunit and mitochondrial small subunit rDNA sequences. Members of these genera have poroid hymenophores, simple septate hyphae and cause brown rots in a variety of substrates. Analyses indicate that Laetiporus and Wolfiporia are not monophyletic. All North American Laetiporus species formed a well Supported monophyletic group (the "core Laetiporus clade" or Laetiporus s.s.) with the exception of L. persicinus, which showed little affinity for an), genus for which sequence data are available. Based on data from GenBank, the southern hemisphere species L. portentosus also fell well outside the core Laetiporus clade. Wolfiporia dilatohypha was found to represent a sister group to the core Laetiporus clade. Isolates of Phaeolus, Pycnoporellus and members of the core Laetiporus clade all fell within the Antrodia clade of polypores, while Leptoporus mollis and Laetiporus portentosus fell within the phlebioid clade of polypores. Wolfiporia cocos isolates also fell in the Antrodia clade, in contrast to previous Studies that. placed W cocos in the core polyporoid clade. ITS analyses resolved eight clades within Laetiporus s.s., three of which might represent undescribed species. A combined analysis using the three DNA regions resolved Five major clades within Laetiporus s.s.: a clade containing conifer-Inhabiting species ("Conifericola clade"), a clade containing L. cincinnatus ("Cincinnatus clade"), a clade containing L. sulphureus s.s. isolates with yellow pores ("Sulphureus clade I"), a clade containing L. sulphureus s.s. isolates with white pores ("Sulphureus clade II") and a clade containing L. gilbertsonii and unidentified isolates from the Caribbean ("Gilbertsonii clade"). Although there is strong support. for groups within the core Laetiporus clade, relationships among these groups remain poorly resolved.