Atomic Force Microscopy Investigation of DNA Extracted from the Vegetative Forms and the Viable but Nonculturable Forms of Mycoplasma gallisepticum S6
Atomic Force Microscopy Investigation of DNA Extracted from the Vegetative Forms and the Viable but Nonculturable Forms of Mycoplasma gallisepticum S6
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DOI：
10.1100/tsw.2008.143
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发表时间：
2008-01-01
影响因子：
--
通讯作者：
Chernov, Vladislav M.
中科院分区：
文献类型：
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作者：
Chernova, Olga A.;Trushin, Maxim V.;Chernov, Vladislav M.
最近的研究表明，支原体有各种各样的生活程序。这意味着一旦这些微生物的环境变得极度改变，形态和基因组表达可能会发生变化。在这篇文章中，我们报告的变化，DNA分子获得的营养形式和可行的，但不可培养（VBNC）形式的鸡毒支原体S6。原子力显微镜研究表明，上述形式的支原体具有不同的DNA参数值（高度：0.461 +/- 0.141和0.236 +/- 0.069 nm;宽度：2.221 +/- 0.286和1.291 +/- 0.705 nm，分别为营养和VBNC形式）。我们认为，观察到的现象可能与这些细菌对恶劣环境的适应过程有关。
Recent studies show that mycoplasmas have various programs of life. This means that changes in morphology and genome expression may occur once the environment of these microorganisms becomes extremely altered. In this article, we report on changes in the DNA molecule obtained from the vegetative forms and the viable but nonculturable (VBNC) forms of Mycoplasma gallisepticum S6. Atomic force microscopy studies show that the above-mentioned forms of the mycoplasma have different values of DNA parameters (height: 0.461 +/- 0.141 and 0.236 +/- 0.069 nm; width: 2.221 +/- 0.286 and 1.291 +/- 0.705 nm for the vegetative and the VBNC forms, respectively). We suppose that the observed phenomenon may be connected with the process of adaptation of these bacteria to severe environments.