REQUIREMENT FOR INTRON-ENCODED U22 SMALL NUCLEOLAR RNA IN 18S RIBOSOMAL-RNA MATURATION
REQUIREMENT FOR INTRON-ENCODED U22 SMALL NUCLEOLAR RNA IN 18S RIBOSOMAL-RNA MATURATION
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DOI：
10.1126/science.7985025
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发表时间：
1994-12-02
期刊：
影响因子：
56.9
通讯作者：
STEITZ, JA
中科院分区：
文献类型：
--
作者：
TYCOWSKI, KT;SHU, MD;STEITZ, JA
脊椎动物细胞的核仁含有许多小RNA，这些小RNA是通过加工蛋白质编码基因转录本的内含子片段而产生的。内含子编码的人类 U22 的宿主基因 (UHG) 很不寻常，因为它指定了一种聚腺苷酸化但显然是非编码的 RNA。通过寡核苷酸引导的核糖核酸酶 H 靶向消除非洲爪蟾卵母细胞中的 U22，阻止了两端 18S 核糖体 RNA (rRNA) 的加工。通过注射体外合成的 U22 RNA 恢复 18S rRNA 的外观。这些结果鉴定了内含子编码的小RNA的细胞功能。
The nucleoli of vertebrate cells contain a number of small RNAs that are generated by the processing of intron fragments of protein-coding gene transcripts. The host gene (UHG) for intron-encoded human U22 is unusual in that it specifies a polyadenylated but apparently noncoding RNA. Depletion of U22 from Xenopus oocytes by oligonucleotide-directed ribonuclease H targeting prevented the processing of 18S ribosomal RNA (rRNA) at both ends. The appearance of 18S rRNA was restored by injection of in vitro-synthesized U22 RNA. These results identify a cellular function for an intronencoded small RNA.