Characterization of tumor angiogenesis in rat brain using iron-based vessel size index MRI in combination with gadolinium-based dynamic contrast-enhanced MRI
Characterization of tumor angiogenesis in rat brain using iron-based vessel size index MRI in combination with gadolinium-based dynamic contrast-enhanced MRI
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DOI：
10.1038/jcbfm.2009.86
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发表时间：
2009-10-01
影响因子：
6.3
通讯作者：
Barbier, Emmanuel L.
中科院分区：
文献类型：
--
作者：
Beaumont, Marine;Lemasson, Benjamin;Barbier, Emmanuel L.
本研究旨在结合基于铁的稳态血管尺寸指数磁共振成像（VSI MRI）方法和基于钆（Gd）的动态对比增强MRI方法（DCE MRI）来表征肿瘤微血管。荷瘤大鼠（C6，n = 14; RG 2，n = 6）在2.35T磁场下，4 h后行DCE MRI及VSI和DCE MRI联合检查。Gd-DOTA（200 μ mol Gd/kg）和超小超顺磁性氧化铁（USPIO）（200 μ mol铁/kg）分别用于DCE和VSI MRI。C6和RG 2胶质瘤对Gd-DOTA的渗透性相同，但血容量分数和VSI不同，与组织学数据一致。USPIO的存在产生了降低的K-反式值。在不存在和存在USPIO的情况下用Gd-DOTA获得的K-反式值与C6胶质瘤良好相关，但与RG 2胶质瘤无关。还观察到，在DCE MRI的时间范围内，USPIO在C6胶质瘤中保持在血管内，而在RG 2胶质瘤中外渗。总之，如果USPIO在DCE MRI实验的时间范围内不外渗，则可以组合VSI和DCE MRI。USPIO外渗起源的机制仍有待阐明。脑血流与代谢杂志（2009）29，1714-1726; doi：10.1038/jcbfm.2009.86; 2009年7月8日在线发表
This study aimed at combining an iron-based, steady-state, vessel size index magnetic resonance imaging (VSI MRI) approach, and a gadolinium (Gd)-based, dynamic contrast-enhanced MRI approach (DCE MRI) to characterize tumoral microvasculature. Rats bearing an orthotopic glioma (C6, n = 14 and RG2, n = 6) underwent DCE MRI and combined VSI and DCE MRI 4 h later, at 2.35 T. Gd-DOTA (200 mu mol of Gd per kg) and ultrasmall superparamagnetic iron oxide (USPIO) (200 mu mol of iron per kg) were used for DCE and VSI MRI, respectively. C6 and RG2 gliomas were equally permeable to Gd-DOTA but presented different blood volume fractions and VSI, in good agreement with histologic data. The presence of USPIO yielded reduced K-trans values. The K-trans values obtained with Gd-DOTA in the absence and in the presence of USPIO were well correlated for the C6 glioma but not for the RG2 glioma. It was also observed that, within the time frame of DCE MRI, USPIO remained intravascular in the C6 glioma whereas it extravasated in the RG2 glioma. In conclusion, VSI and DCE MRI can be combined provided that USPIO does not extravasate with the time frame of the DCE MRI experiment. The mechanisms at the origin of USPIO extravasation remain to be elucidated. Journal of Cerebral Blood Flow & Metabolism (2009) 29, 1714-1726; doi:10.1038/jcbfm.2009.86; published online 8 July 2009