Molecular authentication of geo-authentic Scrophularia ningpoensis
Molecular authentication of geo-authentic Scrophularia ningpoensis
复制标题
地道正宗宁波玄参的分子鉴定
DOI：
10.1631/jzus.b1000179
复制
发表时间：
2011-05-01
影响因子：
5.1
通讯作者：
Fu, Cheng-xin
中科院分区：
文献类型：
--
作者：
Chen, Chuan;Duan, Li-na;Fu, Cheng-xin
玄参在中草药中用于消炎作用由来已久。与其他草药一样，来自不同地区的宁波冬青的疗效可能会有很大差异，而浙江省种植的草药被认为是地道的。然而，通过相似的形态特征很难确定其地理真实性。本研究利用ISSR分子标记对不同来源的宁波沙棘进行了检测。用引物UBC874扩增出1 259个碱基的片段，仅在地道道地标本中发现。通过对该特异条带的克隆和测序，设计了序列特征扩增区(SCAR)标记来区分地道的宁波黄鼠。这是一种快速、简便的方法，可用于鉴定宁波五味子的地理真伪。
Scrophularia ningpoensis has long been used in the Chinese Materia Medica for inflammation. Like other herbal medicines, S. ningpoensis collected from different localities may considerably differ in their therapeutic efficacy, and the one grown in Zhejiang Province is recognized as geo-authentic. However, it is difficult to confirm the geographical authenticity by similar morphological characteristics. In the present study, inter-simple sequence repeat (ISSR) markers were conducted to detect S. ningpoensis from different origins. A 1 259-bp fragment amplified by primer UBC874 was found only in geo-authentic ones. By cloning and sequencing that specific band, sequence characterized amplified region (SCAR) markers were designed to distinguish geo-authentic S. ningpoensis from others. This is a rapid and easy method that can be used to identify the geographical authenticity of S. ningpoensis.