Dynamic holographic endoscopy-ex vivo investigations of malignant tumors in the human stomach
Dynamic holographic endoscopy-ex vivo investigations of malignant tumors in the human stomach
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DOI：
10.1007/s10103-005-0326-1
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发表时间：
2005-04-01
影响因子：
2.1
通讯作者：
Domschke, W
中科院分区：
文献类型：
--
作者：
Avenhaus, W;Kemper, B;Domschke, W
激光全息干涉测量基于物体不同运动状态的全息图在单个全息存储介质上的叠加。通过结合全息干涉测量和内窥镜成像，我们尝试检测胃癌制剂中局部组织弹性紊乱的区域。通过将移动电子散斑干涉测量（ESPI）摄像系统（光源：双频Nd:YAG激光器，λ=532 nm）连接到不同类型的内窥镜，对10个福尔马林固定的人胃进行离体实验，其中9个含有腺癌，1个含有胃淋巴瘤。将内窥镜 ESPI 相机复合体与快速图像处理系统连接起来，以 12.5 Hz 的视频帧速率应用双脉冲曝光图像减法方法。分析了通过用导丝轻轻触摸胃壁变形而产生的散斑相关图案和相应的相位差分布。无肿瘤胃区域在刺激点周围显示高对比度同心条纹。相比之下，所有病例中对应于恶性肿瘤区域的条纹图案和滤波相位差分布均以基本上平行的线为特征，表明刚性肿瘤组织的刺激主要导致倾斜。我们对恶性胃肿瘤的离体研究表明，动态全息内窥镜的应用使得根据组织弹性的差异区分恶性肿瘤区域与周围健康组织成为可能。
Laser holographic interferometry is based on the superimposition of the holograms of different motional states of an object on a single holographic storing medium. Using a combination of holographic interferometry and endoscopic imaging, we tried to detect areas of focally disturbed tissue elasticity in gastric cancer preparations. By connecting a mobile electronic speckle pattern interferometry (ESPI) camera system (light source: double frequency Nd:YAG laser, lambda=532 nm) to different types of endoscopes, ex vivo experiments were performed on ten formalin fixed human stomachs, nine containing adenocarcinomas and one with a gastric lymphoma. Linking the endoscopic ESPI camera complex to a fast image processing system, the method of double pulse exposure image subtraction was applied at a video frame rate of 12.5 Hz. Speckle correlation patterns and corresponding phase difference distributions resulting from gastric wall deformation by gentle touch with a guide wire were analyzed. Tumor-free gastric areas showed high-contrast concentric fringes around the point of stimulation. In contrast, fringe patterns and filtered phase difference distributions corresponding to the areas of malignancy in all the cases were characterized by largely parallel lines, indicating that stimulation of rigid tumor tissue primarily led to tilting. Our ex vivo investigations of malignant gastric tumors show that the application of dynamic holographic endoscopy makes it possible to distinguish areas of malignancy from surrounding healthy tissue based on the differences in tissue elasticity.