Association of CYP1A1 and glutathione S-transferase polymorphisms with male factor infertility
Association of CYP1A1 and glutathione S-transferase polymorphisms with male factor infertility
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DOI：
10.1016/j.fertnstert.2008.07.017
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发表时间：
2009-08-01
影响因子：
6.7
通讯作者：
Aydos, Kaan
中科院分区：
文献类型：
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作者：
Aydos, Sena Erdogan;Taspinar, Mehmet;Aydos, Kaan
目的：研究谷胱甘肽S-转移酶（GST）M1和T1、CYP 1A 1 *2C基因多态性与男性因素不育之间是否存在关系。设计：遗传多态性分析，病例对照研究。设置：大学研究实验室和男科诊所。患者：110名不育男性和105名健康生育男性被纳入研究。干预措施：主要观察指标：采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测CYP 1A 1 *2C、GSTM 1和GSTT 1基因型。结果：单因素分析表明，GSTM 1、CYP 1A 1 *2C基因型与不育症有显著性差异。携带CYP 1A 1瓦尔/瓦尔或CYP 1A 1 Ile/瓦尔与GSTM 1无效基因型相关的受试者发生不育的风险是携带CYP 1A 1 Ile/Ile与GSTM 1野生型基因型相关的受试者的6.90倍（比值比：6.90，95%置信区间：2.29-19.3）。结论：外源性代谢酶基因多态性可能在不育症的发生发展中起重要作用。（Fertil Steril（R）2009;92：541-7. (C)2009年，美国生殖医学会（American Society for Reproductive Medicine）
Objective: To examine whether a relationship exists between genetic polymorphisms of glutathione S-transferase (GST) M1 and T1, CYP1A1*2C, and male factor infertility.Design: Genetic polymorphism analysis, case-control study.Setting: University research laboratory and andrology clinic.Patient(s): One hundred ten men with infertility and 105 healthy fertile men were recruited for the study.Intervention(s): Physical examination of the genitalia of patients, scrotal colored Doppler ultrasound examination, and blood sampling were performed for DNA extraction and genotyping.Main Outcome Measure(s): CYP1A1*2C, GSTM1, and GSTT1 polymorphism genotypes were determined by polymerase chain reaction and restriction fragment length polymorphism methods. Seminal parameters were analyzed.Result(s): There were significant differences between infertility and GSTM1, CYP1A1*2C genotypes by univariate analyses. A subject carrying CYP1A1 Val/Val or CYP1A1 Ile/Val in association with GSTM null genotype has 6.90 times more risk to be infertile than a subject carrying CYP1A1 Ile/Ile in association with GSTM1 wild-type genotype (odds ratio: 6.90, 95% confidence interval: 2.29-19.3). No correlation was found between the seminal parameters and the genetic variability.Conclusion: Our results suggest that genetic polymorphisms of xenobiotic-metabolizing enzymes could play an important role in infertility. (Fertil Steril (R) 2009;92:541-7. (C) 2009 by American Society for Reproductive Medicine.)