Echocardiographic measurement of left ventricular mass and volume in normotensive and hypertensive rats. Necropsy validation.
Echocardiographic measurement of left ventricular mass and volume in normotensive and hypertensive rats. Necropsy validation.
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DOI：
10.1093/ajh/3.9.688
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发表时间：
1990-09
影响因子：
3.2
通讯作者：
G. Simone;D. Wallerson;Massimo Volpe;R. B. Devereux
中科院分区：
文献类型：
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作者：
G. Simone;D. Wallerson;Massimo Volpe;R. B. Devereux
虽然大鼠通常被用来研究左心室(LV)肥厚，但测量LV质量和尺寸需要处死大鼠。为了确定超声心动图在大鼠中的准确性，用立方函数(LVM)或椭圆模型(LVMel)测量了高分辨率M型超声心动图的盲截面积(CSA)和左心室质量(LVMel)，并与41只正常血压大鼠(体重116~762g)和17只高血压大鼠(350~560g)的身体左心室重量(0.28~1.5g)进行了比较。28只正常大鼠的死后心房室体积(0.02至0.19毫升)也与来自椭圆模型的超声心动图体积进行了比较。LVm、CsA和LVMel与LV重量的相关系数分别为r=0.87、0.90和0.93(P均<0.00001)。在高血压大鼠和体重匹配的正常血压大鼠之间的比较显示，LVMel和CsA在检测死后左室肥厚方面的正常上限均为89%的敏感性和100%的特异性。尸检的LV体积与收缩期超声心动图体积的关系比与舒张期体积的关系更密切(r=0.78比0.71，P均小于0.00001)，与死后肌挛缩的影响相一致。5只Wistar大鼠用热稀释法测得的每搏输出量与用椭圆模型回声体积测得的结果相近(0.35+/-0.05比0.30+/-0.06毫升/次)。超声心动图可用于评价大鼠左室结构和功能，以及检测高血压引起的在体左室解剖差异。
Although rats are commonly used to study left ventricular (LV) hypertrophy, measurement of LV mass and dimensions has required killing the rat. To determine the accuracy of echocardiography in rats, blinded crossectional area (CSA) and LV mass measurements using either the cube function (LVM) or an elliptical model (LVMel) from high resolution M-mode echocardiograms were compared to necropsy LV weight (0.28 to 1.5 g), in 41 normotensive (body weight 116 to 762 g) and 17 hypertensive rats (350 to 560 g). Postmortem chamber volumes in 28 normal rats (0.02 to 0.19 mL) were also compared to echocardiographic volumes derived from the elliptical model. Correlation with LV weight was r = 0.87 for LVM, 0.90 for CSA and 0.93 for LVMel (all P less than .00001). Comparison of hypertensive and body-weight-matched normotensive rats revealed the upper normal limit for both LVMel and CSA to have 89% sensitivity and 100% specificity for detection of post mortem LV hypertrophy. Necropsy LV volumes were more closely related to systolic echocardiographic volumes than to diastolic volumes (r = 0.78 v 0.71, both P less than .00001), compatible with the effects of post mortem contracture. Stroke volume determined invasively in 5 Wistar rats by thermodilution was similar to that obtained using elliptical model echo volumes in 5 rats of the same body size (0.35 +/- 0.05 v 0.30 +/- 0.06 mL/beat). Echocardiography can be used to evaluate LV structure and function in rats and to detect in vivo LV anatomic differences induced by hypertension.