Genetic mapping of genes conferring permethrin resistance in the malaria vector, Anopheles gambiae
Genetic mapping of genes conferring permethrin resistance in the malaria vector, Anopheles gambiae
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DOI：
10.1111/j.0962-1075.2004.00495.x
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发表时间：
2004-08-01
影响因子：
2.6
通讯作者：
Collins, FH
中科院分区：
文献类型：
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作者：
Ranson, H;Paton, MG;Collins, FH
冈比亚按蚊是东非主要疟疾病媒，其种群对氯菊酯的抗药性是多因素的。突变的钠通道等位基因和增强的杀虫剂代谢有助于抗性表型。我们使用微卫星标记扫描基因组的数量性状位点（QTL）与氯菊酯抗性。两个主效和一个次效QTL。第一个QTL，rtp1，共定位与染色体2L上的钠通道基因，从而进一步支持该基因的突变赋予氯菊酯抗性的重要性。后两个位点位于第三条染色体上，其中一个，rtp2，侧翼的细胞色素P450基因的大集群。这些区域的进一步详细定位将有助于阐明杀虫剂代谢抗性的分子机制。
Resistance to permethrin in an East African population of the major malaria vector, Anopheles gambiae is multifactorial. A mutated sodium channel allele and enhanced insecticide metabolism contribute to the resistance phenotype. We used microsatellite markers to scan the genome for quantitative trait loci (QTL) associated with permethrin resistance. Two major and one minor QTL were identified. The first QTL, rtp1, colocalizes with the sodium channel gene on chromosome 2L thus further supporting the importance of mutations in this gene in conferring permethrin resistance. The second two loci are located on the third chromosome and one of these, rtp2, flanks a large cluster of cytochrome P450 genes. Further detailed mapping of these regions will help elucidate the molecular mechanisms of metabolic resistance to insecticides.