Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
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DOI：
10.1038/35078107
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发表时间：
2001-05-24
期刊：
影响因子：
64.8
通讯作者：
Tuschl, T
中科院分区：
文献类型：
--
作者：
Elbashir, SM;Harborth, J;Tuschl, T
RNA干扰（RNAi）是动物和植物中序列特异性的转录后基因沉默的过程，由与沉默基因序列同源的双链RNA（dsRNA）启动（1-4）。序列特异性信使RNA降解的介质是由核糖核酸酶III从较长的dsRNA切割产生的21-和22-核苷酸小干扰RNA（siRNA）（5-9）。在这里，我们表明，21个核苷酸的siRNA双链体特异性抑制内源性和异源性基因在不同的哺乳动物细胞系，包括人胚肾（293）和HeLa细胞的表达。因此，21个核苷酸的siRNA双链体为研究哺乳动物细胞中的基因功能提供了一种新的工具，并可能最终用作基因特异性治疗剂。
RNA interference (RNAi) is the process of sequence-specific, post-transcriptional gene silencing in animals and plants, initiated by double-stranded RNA (dsRNA) that is homologous in sequence to the silenced gene(1-4). The mediators of sequence-specific messenger RNA degradation are 21- and 22-nucleotide small interfering RNAs (siRNAs) generated by ribonuclease III cleavage from longer dsRNAs(5-9). Here we show that 21-nucleotide siRNA duplexes specifically suppress expression of endogenous and heterologous genes in different mammalian cell lines, including human embryonic kidney (293) and HeLa cells. Therefore, 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.