CasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivo
CasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivo
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DOI：
10.7554/elife.10936
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发表时间：
2016-04-08
期刊：
影响因子：
7.7
通讯作者：
Montell, Denise J.
中科院分区：
文献类型：
--
作者：
Ding, Austin Xun;Sun, Gongping;Montell, Denise J.
Caspase-3执行细胞凋亡的刽子手阶段，但在特殊情况下，细胞可以存活其活性。为了系统地记录细胞在体内caspase-3激活的位置和时间，我们设计了一个系统CasExpress，它在果蝇中caspase-3激活的细胞中瞬时或永久地驱动荧光蛋白表达。我们发现caspase-3活性的广泛存活。不同的组织出现不同的空间和时间模式。在每个细胞和每个动物的正常发育过程中，一些细胞激活caspase-3，而没有凋亡的证据。在其他组织中，如大脑，表达是零星的时间和空间，并与细胞凋亡的时期重叠。在成年人中，报告基因表达在每只动物的大部分组织中的大部分细胞中是明显的;然而，精确的模式各不相同。抑制翅盘中的半胱天冬酶活性减少翅的大小，证明功能意义。这些模式的影响进行了讨论。
Caspase-3 carries out the executioner phase of apoptosis, however under special circumstances, cells can survive its activity. To document systematically where and when cells survive caspase-3 activation in vivo, we designed a system, CasExpress, which drives fluorescent protein expression, transiently or permanently, in cells that survive caspase-3 activation in Drosophila. We discovered widespread survival of caspase-3 activity. Distinct spatial and temporal patterns emerged in different tissues. Some cells activated caspase-3 during their normal development in every cell and in every animal without evidence of apoptosis. In other tissues, such as the brain, expression was sporadic both temporally and spatially and overlapped with periods of apoptosis. In adults, reporter expression was evident in a large fraction of cells in most tissues of every animal; however the precise patterns varied. Inhibition of caspase activity in wing discs reduced wing size demonstrating functional significance. The implications of these patterns are discussed.