Assessing the diversity of phytotelmic frogs along Madagascar’s east coast: Redefinition of Guibemantis bicalcaratus (BOETTGER, 1913) and revalidation of Guibemantis methueni (ANGEL, 1929)
Assessing the diversity of phytotelmic frogs along Madagascar’s east coast: Redefinition of Guibemantis bicalcaratus (BOETTGER, 1913) and revalidation of Guibemantis methueni (ANGEL, 1929)
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评估马达加斯加东海岸植物性青蛙的多样性：Guibemantis bicalcaratus 的重新定义（BOETTGER，1913）和 Guibemantis methueni 的重新验证（ANGEL，1929）
DOI：
10.3897/vz.63.e31427
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发表时间：
2013
影响因子：
2.1
通讯作者：
C. Raxworthy
中科院分区：
文献类型：
--
作者：
M. Vences;Andolalao Rakotoarison;Andry M Rakotondrazafy;F. Ratsoavina;Roger;F. Glaw;Richard M. Lehtinen;C. Raxworthy
Guibemantis bicalcaratus （Boettger, 1913）被认为是主要在植物上繁殖的马达加斯加青蛙Pandanusicola亚属的一个广泛成员。基于在马达加斯加Nosy Boraha岛和马达加斯加东海岸其他几个地区发现的新标本，我们利用分子和形态学数据修正了bicalcaratus的地位。16S rRNA基因的线粒体DNA序列表明，在Nosy Boraha上存在两个深谱系。其中，迄今为止只在Nosy Boraha发现了一种，而第二种也出现在马达加斯加中部东海岸的其他几个地方。除了一个标本外，两个mtDNA谱系的标本在核RAG1基因序列中没有共享等位基因，这表明尽管同源，但基因流动减少或缺乏，偶尔有杂交。在外部形态上没有发现这两个谱系之间的差异，但在颜色上的微弱差异使我们能够区分大多数标本，因此我们得出结论，它们应该被认为属于两个不同的物种。根据这些颜色差异和与模式标本的比较，我们将只存在于Nosy Boraha的物种分配给Guibemantis bicalcaratus，而第二个物种也出现在Ambila， 1929年Gephyromantis methueni Angel的模式地点。因此，我们复活了这个女人作为这个物种的Guibemantis methueni。这两个物种很可能局限于马达加斯加中部东海岸相对较小的范围，但由于对它们的分布和栖息地需求的基本了解，我们建议它们都处于数据不足的威胁状态。
Guibemantis bicalcaratus (Boettger, 1913) has been considered as a widespread member of the subgenus Pandanusicola of largely phytotelmic-breeding Malagasy frogs. Based on new collections from its type locality, the Malagasy island Nosy Boraha, and several other localities on Madagascar’s east coast, we revise the status of G. bicalcaratus using molecular and morphological data. Mitochondrial DNA sequences of the 16S rRNA gene demonstrate the presence of two deep genealogical lineages of Pandanusicola on Nosy Boraha. Of these, one so far has only been found on Nosy Boraha whereas the second is also present at several other localities along the central east coast of Madagascar. Specimens assigned to the two mtDNA lineages did not share alleles in sequences of the nuclear RAG1 gene except for one specimen, suggesting reduction or absence of gene flow despite sympatry, with occasional hybridization. No differences between the two lineages were found in external morphology but faint differences in color allowed us to discriminate the majority of specimens, and we therefore conclude that they should be considered as belonging to two distinct species. Based on these chromatic differences and comparison with type specimens, we assign the species present only on Nosy Boraha to Guibemantis bicalcaratus whereas the second species also occurs at Ambila, the type locality of Gephyromantis methueni Angel, 1929. We consequently resurrect this nomen as Guibemantis methueni for this species. It is likely that the two species are restricted to relatively small ranges on Madagascar’s central east coast, but due to the rudimentary knowledge on their distribution and habitat requirements we suggest a threat status of Data Deficient for both of them.