Ribosomal RNA genes, RNA polymerases, nucleolar structures, and synthesis of rRNA in the yeast Saccharomyces cerevisiae
Ribosomal RNA genes, RNA polymerases, nucleolar structures, and synthesis of rRNA in the yeast Saccharomyces cerevisiae
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DOI：
10.1101/sqb.2001.66.555
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发表时间：
2001-01-01
期刊：
影响因子：
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通讯作者：
Nomura, M
中科院分区：
文献类型：
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作者：
Nomura, M
556 NOMURA复制叉块（RFB）位点（参见图1的RFB图例）。除了5S RNA基因的存在，大多数真核生物具有相似的重复结构，但具有不同的重复单元大小和不同的重复数，后者范围从小于100到大于10，000（Long和Dawid 1980）。第三，真核细胞有一个清晰的亚核结构，即核仁，rRNA合成和核糖体组装发生在核仁中。除了这些独特的特征外，真核细胞显然具有将细胞核与细胞质分开的核膜。这一事实提出了r蛋白输入和核糖体输出的问题，这可能会以某种方式影响rDNA的转录，但我们的讨论主要限于与Pol I转录35S rRNA基因直接相关的特征。
556 NOMURA replication fork block (RFB) site (see the legend to Fig. 1 for RFB). Except for the presence of the 5S RNA gene, most eukaryotes have similar repeat structures, but with various sizes for a repeat unit and with various repeat numbers, the latter ranging from less than 100 to more than 10,000 (Long and Dawid 1980). Third, eukaryotic cells have a clearly recognized subnuclear structure, the nucleolus, where rRNA synthesis and ribosome assembly take place. In addition to these unique features, eukaryotic cells obviously have a nuclear membrane separating the nucleus from the cytoplasm. This fact poses the questions of r-protein import and ribosome export, which might somehow influence rDNA transcription, but we limit our discussion mainly to features directly related to transcription of the 35S rRNA gene by Pol I.