Microarray analysis of diet-induced alterations in gene expression in the ACI rat prostate.
Microarray analysis of diet-induced alterations in gene expression in the ACI rat prostate.
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发表时间：
2002-08
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通讯作者：
N. Reyes;M. Iatropoulos;A. Mittelman;J. Geliebter
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作者：
N. Reyes;M. Iatropoulos;A. Mittelman;J. Geliebter
前列腺癌的自然病程是一个多阶段的过程，包括从正常组织到亚临床癌症的转变，并发展为原位癌，最终转移性疾病。有证据表明，高脂肪饮食在该疾病的生物学和进展中起着关键作用。ACI大鼠饲养两代，分别饲喂高脂或对照饲料18个月。采用Affymetrix微阵列分析不同饮食大鼠前列腺背外侧mRNA的表达水平。两种饮食的大鼠前列腺中大约有4752个基因和表达序列标签（EST）表达。27个基因在高脂肪饮食中上调，28个基因下调。数据分析表明，高脂肪牛肉饮食影响炎症、葡萄糖和脂肪酸代谢、雄激素代谢、潜在肿瘤抑制和蛋白激酶活性相关基因的表达，以及细胞内和细胞外基质分子、生长因子和雄激素应答基因的表达。这些和未来研究的结果将有助于更好地了解饮食对前列腺基因表达的影响，并有助于合理设计和评估预防前列腺癌的潜在饮食方案。
The natural history of prostate cancer is a multistage process that involves the transition from normal tissue to subclinical cancer, with progression to carcinoma in situ and eventually metastatic disease. Evidence suggests that a high-fat diet plays a critical role in the biology and progression of the disease. ACI rats were maintained for two generations on high beef fat or control diets for 18 months. Affymetrix microarrays were used to analyze the mRNA expression levels in the dorsolateral prostates of rats on the different diets. Approximately 4752 genes and expressed sequence tag (EST) were expressed in the prostates of rats on either diet. Twenty-seven genes were upregulated and 28 genes downregulated in the high beef fat diet. Data analysis indicated that a high beef fat diet affects the expression of genes involved in inflammation, glucose and fatty acid metabolism, androgen metabolism, potential tumor suppression and protein kinase activity, as well as intracellular and extracellular matrix molecules, growth factors and androgen responsive genes. Results from these and future studies will lead to a better understanding of the effect of diet on gene expression in the prostate and facilitate the rational design and assessment of potential dietary programs for prostate cancer prevention.