Fibroblast receptor for cell-substratum adhesion: studies on the interaction of baby hamster kidney cells with latex beads coated by cold insoluble globulin (plasma fibronectin).
Fibroblast receptor for cell-substratum adhesion: studies on the interaction of baby hamster kidney cells with latex beads coated by cold insoluble globulin (plasma fibronectin).
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DOI：
10.1083/jcb.86.1.104
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发表时间：
1980-07
期刊：
影响因子：
--
通讯作者：
Grinnell F
中科院分区：
文献类型：
--
作者：
Grinnell F
对不带电乳胶珠（0.76微米）与幼仓鼠肾细胞的相互作用进行了研究。如果珠被冷不溶性球蛋白（CIG）（血浆纤连蛋白）包被，则珠与细胞结合，但如果珠被牛白蛋白包被，则不结合。即使在孵育培养基中存在过量CIG的情况下，牛白蛋白包被的珠也不与细胞结合。珠粒的结合在悬浮液中的细胞的整个表面上随机发生。然而，细胞受体的CIG珠不再检测上表面的细胞传播上CIG包被的组织培养皿。CIG珠与细胞的结合发生在从4 ° C至37 ° C的所有测试温度下，但速率在4 ° C下最低。在37 ℃时，结合伴随着内吞作用，并且在似乎是溶酶体的囊泡内发现珠粒。在37 ℃下与细胞孵育期间，放射性标记的CIG珠也释放放射性。CIG珠与细胞的结合不需要二价阳离子。最后，细胞胰蛋白酶消化后，CIG珠的细胞受体丢失。数据进行了讨论，在目前的想法细胞粘附的基础。
Studies were carried out on the interactions of uncharged latex beads (0.76 micrometer) with baby hamster kidney cells. Binding of beads to the cells occurred if the beads were coated by cold insoluble globulin (CIG) (plasma fibronectin) but not if the beads were coated by bovine albumin. Bovine albumin-coated beads did not bind to the cells even in the presence of excess CIG in the incubation medium. Binding of beads occurred randomly over the entire surfaces of cells in suspension. However, cell receptors for CIG beads were no longer detectable on the upper surface of cells spread onCIG-coated tissue culture dishes. Binding of CIG beads to cells occurred at all temperatures tested from 4 degrees to 37 degrees C but the rate was lowest at 4 degrees C. At 37 degrees C, binding was accompanied by endocytosis and the beads were found inside vesicles which appeared to be lysosomes. There was also release of radioactivity from radiolabeled CIG beads during incubation with the cells at 37 degrees C. Binding of CIG beads to cells did not require divalent cations. Finally, the cell receptor for CIG beads was lost after cell trypsinization. The data are discussed in terms of current ideas about the basis for cell adhesion.