GENETIC IDENTIFICATION OF ENDOGENOUS POLYTROPIC PROVIRUSES BY USING RECOMBINANT INBRED MICE
GENETIC IDENTIFICATION OF ENDOGENOUS POLYTROPIC PROVIRUSES BY USING RECOMBINANT INBRED MICE
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DOI：
10.1128/jvi.63.9.3810-3821.1989
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发表时间：
1989-09-01
影响因子：
5.4
通讯作者：
COFFIN, JM
中科院分区：
文献类型：
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作者：
FRANKEL, WN;STOYE, JP;COFFIN, JM
通过对重组近交系（RI）小鼠原病毒-细胞DNA功能片段分离的检测，鉴定出47种内源性嗜多性鼠病毒（Pmv）。大多数Pmv基因座被发现在一个以上的七RI祖株分析，但只有四个存在于所有菌株。41个pmv基因座的染色体分配，通过比较其RI株分布模式与已知的遗传标记。Pmv基因座分散在整个基因组中，第1、3、4、5、7、11、12、15和16号染色体各携带三个或更多个前病毒。AKXD RI连锁分析表明，DBA/2 J小鼠的水貂细胞灶形成病毒抗性（RcmfT）基因可能不是Pmv原病毒。还推断，不需要单一的AKR/J特异性Pmv前病毒作为7号染色体上白化病突变的env基因供体。
Forty-seven endogenous polytropic murine viruses (Pmv) were identified by examination of proviral-cellular DNA function fragment segregation in recombinant inbred (RI) mice. Most Pmv loci were found in more than one of seven RI progenitor strains analyzed, but only four were present in all strains. Chromosomal assignments for 41 pmv loci were determined by comparing their RI strain distribution patterns with those of known genetic markers. Pmv loci were found dispersed throughout the genome, with chromosomes 1, 3, 4, 5, 7, 11, 12, 15, and 16 each carrying three or more proviruses. Linkage analysis in the AKXD RI set suggested that the gene encoding mink cell focus-forming virus resistance (RcmfT) of DBA/2J mice is probably not a Pmv provirus. It was also deduced that no single, AKR/J-specific Pmv provirus is required as an env gene donor for albino mutation on chromosome 7.