Investigation of the Long-Term Antibacterial Properties of Titanium by Two-Step Micro-Arc Oxidation Treatment
Investigation of the Long-Term Antibacterial Properties of Titanium by Two-Step Micro-Arc Oxidation Treatment
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DOI：
10.3390/coatings11070798
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发表时间：
2021-07
期刊：
影响因子：
3.4
通讯作者：
H. Tsutsumi;Y. Tsutsumi;Masaya Shimabukuro;Tomoyo Manaka;Peng Chen;M. Ashida;K. Ishikawa;H. Katayama;T. Hanawa
中科院分区：
文献类型：
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作者：
H. Tsutsumi;Y. Tsutsumi;Masaya Shimabukuro;Tomoyo Manaka;Peng Chen;M. Ashida;K. Ishikawa;H. Katayama;T. Hanawa
近年来，细菌黏附和定植引起的生物膜形成已被认为是骨科和牙科种植手术失败的主要原因。在这项研究中，开发了一种定制的微弧氧化(MAO)处理技术，以获得理想的钛表面抗菌性能。采用两步微弧氧化工艺制备了表面氧化层中含银和不含锌的样品。为了模拟实际应用，通过表面分析和浸泡试验，分别评估了银和锌在氧化层中的掺杂情况和离子释放行为。此外，采用革兰氏阴性兼性厌氧菌对长期浸泡在生理盐水中的样品的抗菌性能进行了评价。含有银和锌的MAO处理后的样品对大肠杆菌具有良好的抗菌性能，即使在模拟实际使用的生理盐水中浸泡6个月后也能保持这种抗菌性能。此外，从表面氧化物释放的银离子表明了样品的早期抗菌性能，而锌的腐蚀产物的释放则表明了后期的抗菌性能。
Recently, biofilm formation caused by bacterial adhesion and colonization has been recognized as the major cause of failure in orthopedic and dental implant surgeries. In this study, a customized micro-arc oxidation (MAO) treatment technique was developed to obtain desirable antibacterial properties on Ti surfaces. The two-step MAO treatment was applied in the fabrication of specimens with Ag and with/without Zn in their surface oxide layer. In order to simulate practical usage, surface analyses and immersion tests were performed to evaluate the incorporation of Ag and Zn into the resulting oxide layer and ion release behavior, respectively. Additionally, the antibacterial properties of the specimens after long-term immersion in physiological saline were evaluated using Gram-negative facultative anaerobic bacteria. The MAO-treated specimens containing Ag and Zn exhibited excellent antibacterial properties against Escherichia coli, which were sustained even after 6 months of immersion in physiological saline to simulate practical usage. Moreover, the Ag ions released from the surface oxide indicate the antibacterial properties of the specimen in the early stage, while the release of the corrosion products of Zn demonstrates its antibacterial properties in the later stage.