Dielectrophoresis-based cell manipulation using electrodes on a reusable printed circuit board
Dielectrophoresis-based cell manipulation using electrodes on a reusable printed circuit board
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DOI：
10.1039/b904328d
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Bashir, Rashid
中科院分区：
文献类型：
--
作者：
Park, Kidong;Suk, Ho-Jun;Bashir, Rashid
基于介电电泳的粒子操纵技术在细胞图案化和组织工程等领域有着广泛的应用前景。尽管存在关于使用DEP用于细胞图案化应用的广泛报道，但仍然期望开发使DEP甚至更可负担和更常见的方法。在这项研究中，我们提出了使用的印刷电路板（PCB）上的叉指电极，可以重复使用的操作和位置HeLa细胞和聚苯乙烯颗粒超过100 μ m厚的玻璃盖玻片，使用DEP。将均由PDMS制成的开放孔或封闭微流体通道放置在玻璃盖玻片上，然后将其直接放置在PCB上。将AC电压施加到PCB上的电极以通过薄玻璃盖玻片在颗粒上诱导DEP。随后生长用DEP图案化的HeLa细胞以确认电场没有任何不利影响。这种用于DEP粒子操作的替代和可重复使用的平台可以提供用于原型化基于DEP的芯片实验室系统、成本敏感的芯片实验室应用和广泛的组织工程应用的方便和快速的方法。
Particle manipulation based on dielectrophoresis (DEP) can be a versatile and useful tool in lab-on-chip systems for a wide range of cell patterning and tissue engineering applications. Even though there are extensive reports on the use of DEP for cell patterning applications, the development of approaches that make DEP even more affordable and common place is still desirable. In this study, we present the use of interdigitated electrodes on a printed circuit board (PCB) that can be reused to manipulate and position HeLa cells and polystyrene particles over 100 mu m thick glass cover slips using DEP. An open-well or a closed microfluidic channel, both made of PDMS, was placed on the glass coverslip, which was then placed directly over the PCB. An AC voltage was applied to the electrodes on the PCB to induce DEP on the particles through the thin glass coverslip. The HeLa cells patterned with DEP were subsequently grown to confirm the lack of any adverse affects from the electric fields. This alternative and reusable platform for DEP particle manipulation can provide a convenient and rapid method for prototyping a DEP-based lab-on-chip system, cost-sensitive lab-on-chip applications, and a wide range of tissue engineering applications.