Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: their prognostic value and role in tumour invasion and progression
Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: their prognostic value and role in tumour invasion and progression
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DOI：
10.1016/s0959-8049(02)00210-1
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发表时间：
2002-12-01
影响因子：
8.4
通讯作者：
Pavlidis, N
中科院分区：
文献类型：
--
作者：
Ioachim, E;Charchanti, A;Pavlidis, N
对134例可手术乳腺癌组织中细胞外基质（ECM）成分腱粘蛋白（TN）、纤维连接蛋白（FN）、IV型胶原（科尔）和层粘连蛋白（LN）的表达进行了免疫组化研究。它们的表达也与蛋白水解酶组织蛋白酶D（CD），粘附分子CD 44标准形式（CD 44 s）和其他已知因子的表达进行了比较，以阐明这些分子在肿瘤进展和转移中的预后价值和作用。TN在肿瘤间质中的表达与肿瘤分级和大小、CD 44 s表达、肿瘤和间质CD表达以及FN、层粘连蛋白和科尔在相同区域的表达呈正相关。TN表达与ER状态呈负相关。其在浸润前沿的表达仅与淋巴结状态呈正相关。生存分析显示高水平的TN表达增加了死亡风险。在多变量分析中，在ECM蛋白中，只有TN表达与患者的生存独立相关。FN表达与淋巴结转移、增殖相关指数Ki-67和间质CD表达呈正相关。生存分析显示高水平FN表达与死亡风险增加相关。科尔蛋白表达与肿瘤大小及LN表达呈正相关。科尔表达与ER和PgR受体状态呈负相关，LN表达与肿瘤和间质CD表达、增殖相关指数Ki-67呈正相关，与ER受体状态呈负相关。在乳腺癌组织中观察到的ECM蛋白表达的改变及其与蛋白水解酶CD和粘附分子CD 44 s的相关性，表明参与癌症进展。此外，间质TN和FN的过度表达似乎在乳腺癌患者中具有负面的预后价值。(C)2002爱思唯尔科技有限公司版权所有。
The immunohistochemical expression of the extracellular matrix (ECM) components tenascin (TN), fibronectin (FN), collagen type IV (Coll) and laminin (LN), and their possible relationships were studied in a series of 134 operable breast cancer cases. Their expression was also compared with the expression of the proteolytic enzyme cathepsin D (CD), the adhesion molecule CD44 standard form (CD44s) and other known factors to clarify the prognostic value and role of these molecules in tumour progression and metastasis. TN expression in the tumour stroma was positively correlated with tumour grade and size, CD44s expression, tumour and stromal CD expression as well as with FN, laminin and Coll expression in the same areas. TN expression was inverse correlated with ER status. Its expression at the invasion front was only positively correlated with the lymph node status. Survival analysis showed an increased mortality risk associated with high levels of TN expression. In multivariate analysis, among the ECM proteins, only TN expression was independently correlated with patients' survival. FN expression was positively correlated with lymph node involvement, with the proliferation-associated index Ki-67 and stromal CD expression. Survival analysis showed an increased mortality risk associated with a high level of FN expression. Coll expression was positively correlated with the tumour size and LN expression. An inverse relationship of Coll expression with ER and PgR receptor status was also found. LN expression was positively correlated with tumour and stromal CD expression, with the proliferation-associated index Ki-67 and inversely with ER receptor status. The observed alterations in the expression of ECM proteins in breast cancer tissue and their correlations with the proteolytic enzyme CD and the adhesion molecule CD44s, suggest an involvement in cancer progression. In addition, overexpression of stromal TN and FN seems to have negative prognostic value in breast cancer patients. (C) 2002 Elsevier Science Ltd. All rights reserved.