Identification of new genetic regions more prevalent in nontypeable Haemophilus influenzae otitis media strains than in throat strains.
Identification of new genetic regions more prevalent in nontypeable Haemophilus influenzae otitis media strains than in throat strains.
复制标题
鉴定新基因区域在不可分型流感嗜血杆菌中耳炎菌株中比在咽喉菌株中更为普遍。
DOI：
10.1128/jcm.01331-06
复制
发表时间：
2006
影响因子：
9.4
通讯作者：
Marrs,CarlF
中科院分区：
文献类型：
--
作者：
Xie,Jingping;Juliao,PatriciaC;Gilsdorf,JanetR;Ghosh,Debashis;Patel,Mayuri;Marrs,CarlF
不可分型（NT）流感嗜血杆菌菌株引起严重的呼吸系统疾病，并从急性中耳炎患儿多达一半的中耳吸出物中分离出来。先前的研究已经确定了与NTH相关的两个基因lic2BandhmwA。从患有中耳炎的儿童中耳分离出的流感菌株，但与NTH无关。从健康儿童喉咙中分离出的流感菌株，提示它们可能在中耳炎的毒力中起作用。在这项研究中，基因组减法被用来鉴定中耳菌株特有的额外遗传区域。NTH的基因组。从NTH中减去流感中耳菌株G622。鉴定了流感喉株23221和中耳株所特有的基因区域。随后，中耳特异性基因区域在大量中耳炎和喉炎菌株中的相对流行率通过斑点杂交确定。通过这种方法，发现9个遗传区域在中耳炎菌株中更为普遍。对flic2b、hmwA和9个新的中耳炎潜在毒力基因进行分类树分析，发现2个oh。与中耳炎相关的流感病原型。
Nontypeable (NT)Haemophilus influenzaestrains cause significant respiratory illness and are isolated from up to half of middle ear aspirates from children with acute otitis media. Previous studies have identified two genes,lic2BandhmwA, that are associated with NTH. influenzaestrains isolated from the middle ears of children with otitis media but that are not associated with NTH. influenzaestrains isolated from the throats of healthy children, suggesting that they may play a role in virulence in otitis media. In this study, genomic subtraction was used to identify additional genetic regions unique to middle ear strains. The genome of NTH. influenzaemiddle ear strain G622 was subtracted from that of NTH. influenzaethroat strain 23221, and the resultant gene regions unique to the middle ear strain were identified. Subsequently, the relative prevalence of the middle ear-specific gene regions among a large panel of otitis media and throat strains was determined by dot blot hybridization. By this approach, nine genetic regions were found to be significantly more prevalent in otitis media strains. Classification tree analysis oflic2B,hmwA, and the nine new potential otitis media virulence genes revealed twoH. influenzaepathotypes associated with otitis media.