On-demand quantitative SERS bioassays facilitated by surface-tethered ratiometric probes
On-demand quantitative SERS bioassays facilitated by surface-tethered ratiometric probes
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由表面系留比率探针促进的按需定量 SERS 生物测定
DOI：
10.1039/c8sc03263g
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发表时间：
2018
期刊：
影响因子：
8.4
通讯作者：
Liu Baohong
中科院分区：
文献类型：
--
作者：
Zhang Kun;Wang Yuning;Wu Meiling;Liu Yujie;Shi Dongyun;Liu Baohong
对复杂生物流体中的目标分析物进行可靠且用户友好的传感对于生物科学和医学至关重要。表面增强拉曼光谱（SERS）已被证明能够以高灵敏度检测分子，但实现稳健的定量检测仍然是一个挑战，主要是因为电磁热点处的严重信号波动。在这里，我们描述了一种基于芯片的传感装置的代谢物分析的按需和定量 SERS 策略，该传感装置采用稳定的表面束缚小分子探针作为拉曼报告基因。这些具有比例响应的探针通过提供内部校准来校正由测定条件的时空变化引起的信号波动，从而实现灵敏且可重复的 SERS 检测。同时，基于芯片的传感方案使得分时按需检测成为可能。最终，由于选择不同比例拉曼探针的灵活性，我们期望所提出的定量 SERS 传感概念对细胞生物学和临床诊断领域的研究有用。
Reliable and user-friendly sensing of target analytes in complex biofluids is of fundamental importance to biological science and medicine. Surface-enhanced Raman spectroscopy (SERS) has proven to be capable of detecting molecules with high sensitivity, but achieving robust quantitative detection remains a challenge mainly because of the severe signal fluctuation at electromagnetic hot spots. Here, we describe an on-demand and quantitative SERS strategy for metabolite profiling based on a chip-based sensing device that adopts stable and surface-tethered small-molecule probes as Raman reporters. These probes with a ratiometric response allow for sensitive and reproducible SERS detection by offering an internal calibration to correct the signal fluctuation caused by the spatiotemporal variation of assay conditions. Meanwhile, the chip-based sensing scheme makes time-separated on-demand detection possible. Ultimately, due to the flexibility in choosing diverse ratiometric Raman probes, we expect the proposed quantitative SERS sensing concept to be useful for studies in the fields of cell biology and clinical diagnosis.