Geographical genetic structure within the human lung fluke, Paragonimus westermani, detected from DNA sequences
Geographical genetic structure within the human lung fluke, Paragonimus westermani, detected from DNA sequences
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DOI：
10.1017/s0031182097001534
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发表时间：
1997-10-01
期刊：
影响因子：
2.4
通讯作者：
Ito, A
中科院分区：
文献类型：
--
作者：
Blair, D;Agatsuma, T;Ito, A
从日本、中国、韩国、台湾、菲律宾、马来西亚半岛和泰国的卫氏并殖吸虫地理分离株中获得核糖体基因重复序列的第二个内转录间隔区和线粒体细胞色素c氧化酶亚基I基因的一部分的核苷酸序列。从并殖吸虫的几个其他物种中获得序列用于比较目的。在卫氏肺吸虫中可识别出两个类群：一个是相对统一的东北类群（中国、日本、韩国、台湾），包括二倍体和三倍体形式;另一个是南方类群（马来西亚、泰国、菲律宾），其成员之间的遗传距离较远。根据ITS 2和COI数据，卫氏并殖吸虫分离株之间的遗传距离等于或超过了一些不同种的并殖吸虫之间的遗传距离。ITS 2序列相对于COI序列是保守的。在并殖吸虫属内，后者之间的替代可能接近饱和。
Nucleotide sequences were obtained for the second internal transcribed spacer of the ribosomal gene repeat and for part of the mitochondrial cytochrome c oxidase subunit I gene from geographical isolates of Paragonimus westermani from Japan, China, Korea, Taiwan, the Philippines, peninsular Malaysia and Thailand. Sequences were obtained from several other species of Paragonimus for comparative purposes. Two groups were recognized within P. westermani: an NE group (China, Japan, Korea, Taiwan) which was relatively uniform and included both diploid and triploid forms, and a southern group (Malaysia, Thailand, Philippines), members of which were genetically distant from one another. According to both ITS2 and COI data, genetic distances among P. westermani isolates equalled or exceeded those between some distinct species of Paragonimus. The ITS2 sequences were conserved relative to COI sequences. Substitutions among the latter may be approaching saturation within the genus Paragonimus.