Adsorption of high density lipoproteins (HDL) on solid surfaces
Adsorption of high density lipoproteins (HDL) on solid surfaces
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DOI：
10.1006/jcis.1996.0045
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发表时间：
1996-02-10
影响因子：
9.9
通讯作者：
Greve, J
中科院分区：
文献类型：
--
作者：
Feng, M;Morales, AB;Greve, J
研究了高密度脂蛋白(HDL)在聚乙烯(PE)、聚甲基丙烯酸羟乙酯(PEMA)、聚酯氨酯(PU)、生物体和云母表面的吸附行为。高密度脂蛋白在单一蛋白质溶液和血浆溶液表面的吸附表明，高密度脂蛋白的吸附量与表面的疏水性(或亲水性)无关。实验结果表明，随着单一蛋白质溶液中高密度脂蛋白浓度的增加，高密度脂蛋白在PE上的吸附量逐渐增加，直至5µg/ml时，随着血浆浓度的增加，高密度脂蛋白在PE上的吸附量也随之增加。此外，在25℃下，从浓度为500微克/毫升的高密度脂蛋白溶液中的高密度脂蛋白在PE上的吸附在几分钟内达到最大值，只有一定比例的高密度脂蛋白在与吐温20或十二烷基硫酸钠(SDS)孵育时才能脱附，而脱附取决于表面的性质。解吸从血浆中吸附到PE上的高密度脂蛋白比解吸从单一蛋白质溶液中吸附到PE上的高密度脂蛋白更难。结果表明，当蛋白质层在缓冲液(pH 7.4)中保存24 h后，洗涤剂对吸附在PE上的高密度脂蛋白的解吸作用降低，而较高的保存温度对脂蛋白的解吸有负面影响。用轻拍模式原子力显微镜(AFM)对生理缓冲液中吸附在云母上的高密度脂蛋白进行成像。表面似乎覆盖着单一的高密度脂蛋白以及由两到三个平均高度为5-6 nm的高密度脂蛋白组成的簇。此外，AFM测量证实了吸附在云母上的高密度脂蛋白的部分脱附。(C)1996年学术出版社。
The adsorption of high density lipoproteins (HDL) on polyethylene (PE), poly(2-hydroxyethyl methacrylate) (poly(HEMA)), polyesterurethane (PU), Biomer, and mica surfaces was studied. The adsorption of HDL from a single protein solution and a plasma solution on the surfaces showed that the amount of adsorbed HDL was not related to the hydrophobicity (or hydrophilicity) of the surfaces. It was observed that the amount of HDL adsorbed on PE increased with increasing HDL concentration of a single protein solution until 5 mu g/ml, and increasing plasma concentration resulted in an increase of HDL adsorption. In addition, HDL adsorption from an HDL solution of 500 mu g/ml on PE reached a maximum within a few minutes at 25 degrees C. Only a propertion of adsorbed HDL could be desorbed when the adsorbed layers were incubated with Tween 20 or sodium dodecyl sulfate (SDS), while the desorption was dependent on the nature of the surfaces. It was more difficult to desorb HDL adsorbed from plasma to PE than to desorb HDL adsorbed from a single protein solution to PE. It was found that the desorption of adsorbed HDL from PE by the detergents was decreased if the protein layer had been stored in buffer (pH 7.4) for 24 h before desorption, while a higher storing temperature had a negative effect on the desorption of the lipoprotein from the surface. Adsorbed HDL on mica in a physiological buffer was imaged by a tapping mode atomic force microscope (AFM). The surface appeared to be covered by single HDL proteins as well as clusters of two or three HDL proteins with an average height of 5 to 6 nm. Furthermore, the partial desorption of adsorbed HDL from mica was confirmed by AFM measurements. (C) 1996 Academic Press, Inc.