Crystallization and preliminary X-ray study of endopolygalacturonase from the pathogenic fungus Stereum purpureum.
Crystallization and preliminary X-ray study of endopolygalacturonase from the pathogenic fungus Stereum purpureum.
复制标题
病原真菌 Stereum purpureum 内聚半乳糖醛酸酶的结晶和初步 X 射线研究。
DOI：
10.1107/s0907444901009568
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Hiroaki Kato
中科院分区：
文献类型：
--
作者：
Tetsuya Shimizu;T. Nakatsu;K. Miyairi;T. Okuno;Hiroaki Kato
用气相扩散法从紫柄革菌中获得了内切多聚半乳糖醛酸酶I的晶体。在结晶工作之前，用内切-β-N-乙酰氨基葡糖苷酶H将内切多聚半乳糖醛酸酶I去糖基化。该晶体使用同步辐射衍射至λ（0.96 A）分辨率，属于空间群P1，晶胞参数a = 37.26，B = 46.34，c = 52.05 A，α = 67.17，β = 72.44，γ = 68.90度。
Crystals of endopolygalacturonase I from Stereum purpureum have been obtained by the vapour-diffusion method. Prior to crystallization work, endopolygalacturonase I was deglycosylated with endo-beta-N-acetylglucosaminidase H. The crystal diffracts to ultrahigh (0.96 A) resolution using synchrotron radiation and belongs to space group P1, with unit-cell parameters a = 37.26, b = 46.34, c = 52.05 A, alpha = 67.17, beta = 72.44, gamma = 68.90 degrees.