Dominant negative c-Src inhibits angiotensin II induced activation of NHE3 in OKP cells.
Dominant negative c-Src inhibits angiotensin II induced activation of NHE3 in OKP cells.
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显性负性 c-Src 抑制 OKP 细胞中血管紧张素 II 诱导的 NHE3 激活。
DOI：
10.1046/j.1523-1755.1998.00029.x
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发表时间：
1998
影响因子：
19.6
通讯作者：
Alpern,RJ
中科院分区：
文献类型：
--
作者：
Tsuganezawa,H;Preisig,PA;Alpern,RJ
Dominant negative c-Src inhibits angiotensin II induced activation of NHE3 in OKP cells.BackgroundAngiotensin II is a potent stimulator of the proximal tubule apical membrane Na/H antiporter, encoded by NHE3. The nonreceptor tyrosine kinase, c-Src, plays a key role in regulation of NHE3 by acidosis in the proximal tubule, and in signaling effects of angiotensin II in vascular smooth muscle.MethodsThe present studies examined the role of c-Src in mediating angiotensin II-induced NHE3 activation in cultured OKP cells. c-Src was inhibited with herbimycin A, a tyrosine kinase inhibitor, and expression of a dominant negative c-Src, c-SrcK295M.ResultsHerbimycin A blocked angiotensin II induced increases in Na/H antiporter activity. In two clonal cell lines expressing vector alone, angiotensin II increased Na/H antiporter activity, while in three clones expressing c-SrcK295M, angiotensin II had no effect. Cyclic AMP and protein kinase A have been proposed to be key mediators in regulation of NHE3 by angiotensin II. 10-4m8-bromo cAMP induced a 40 to 50% inhibition of Na/H antiporter activity in cells expressing c-SrcK295M, similar to that seen in wild-type OKP cells. In addition, cells expressing c-SrcK295Mresponded normally to 10-7mdexamethasone with a 50 to 80% increase in Na/H antiporter activity.ConclusionsThese studies demonstrate that c-Src is required for angiotensin II-induced increases in NHE3 activity. Thus, c-Src plays a key role in antiporter activation by acidosis and angiotensin II.
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DOI：
10.1073/pnas.87.20.7917
发表时间：
1990-10-01
影响因子：
11.1
作者：
GEIBEL, J;GIEBISCH, G;BORON, WF
通讯作者：
BORON, WF
与近端肾小管上皮血管紧张素受体偶联的信号传导机制。
DOI：
--
发表时间：
1990
期刊：
Kidney international. Supplement
影响因子：
--
作者：
Douglas,JG;Romero,M;Hopfer,U
通讯作者：
Hopfer,U
在 OKP 细胞中，血管紧张素 II 对 Na-H 逆向转运蛋白活性的刺激与 cAMP 无关。
DOI：
10.1152/ajpcell.1994.266.6.c1603
发表时间：
1994
期刊：
The American journal of physiology
影响因子：
--
作者：
Cano,A;Miller,RT;Alpern,RJ;Preisig,PA
通讯作者：
Preisig,PA
DOI：
10.1152/ajpcell.1996.271.3.c763
发表时间：
1996-09-01
影响因子：
5.5
作者：
Chu, TS;Tsuganezawa, H;Alpern, RJ
通讯作者：
Alpern, RJ
G 蛋白偶联受体通过 pp60c-src 和 p21ras 控制血管平滑肌细胞增殖。
DOI：
10.1152/ajpcell.1997.272.6.c2019
发表时间：
1997
期刊：
The American journal of physiology
影响因子：
--
作者：
Schieffer,B;Drexler,H;Ling,BN;Marrero,MB
通讯作者：
Marrero,MB