Effects of local administration of vascular endothelial growth factor on properties of the in situ frozen-thawed anterior cruciate ligament in rabbits
Effects of local administration of vascular endothelial growth factor on properties of the in situ frozen-thawed anterior cruciate ligament in rabbits
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DOI：
10.1177/0363546505278700
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发表时间：
2006-01-01
影响因子：
4.8
通讯作者：
Yasuda, K
中科院分区：
文献类型：
--
作者：
Ju, YJ;Tohyama, H;Yasuda, K
背景：在用于前交叉韧带重建的自体肌腱中，内在成纤维细胞在手术后立即坏死，并发生再增殖和血运重建。血管内皮生长因子被认为是血管生成的有效介质。假设：血管内皮生长因子的应用显着增强原位冷冻前十字韧带的血管生成，并且该应用显着影响原位冷冻前十字韧带的机械性能。研究设计：对照实验室研究。方法：对 66 只兔子的右前十字韧带进行了血管内皮生长因子的研究。冻融处理，然后将动物分为3组。第一组作为冻融但未经其他处理的对照。在第II组中，仅应用0.2mL磷酸盐缓冲盐水。 III组应用30μg血管内皮生长因子。在组织学血运重建检查的基础上对各组进行比较，使用 Chalkley 评分（微血管密度指标）和机械评估，其中包括在前后负载 +/- 10 N 期间胫骨相对于股骨的前后平移以及前十字韧带前内侧束的机械性能。 结果：第 III 组的 Chalkley 评分显着高于第 I 组和第 II 组。 Ⅰ、Ⅱ、Ⅲ组前交叉韧带的拉伸强度和切线模量显着低于正常前交叉韧带，但Ⅰ、Ⅱ、Ⅲ组之间无显着性差异。结论：本研究给予血管内皮生长因子，原位冻融处理后可显着促进失活前交叉韧带的血管生成，但不影响力学性能。兔模型中原位冻融前十字韧带的特性。临床相关性：重组前十字韧带的应用是增强前十字韧带重建中自体移植物血运重建的潜在未来策略。
Background: In the autogenous tendon for anterior cruciate ligament reconstruction, intrinsic fibroblasts are necrotized immediately after surgery, and repopulation and revascularization occur. Vascular endothelial growth factor is considered to be a potent mediator of angiogenesis.Hypothesis: An application of vascular endothelial growth factor significantly enhances angiogenesis in the in situ frozen anterior cruciate ligament, and the application significantly affects mechanical properties of the in situ frozen anterior cruciate ligament.Study Design: Controlled laboratory study.Methods: Right anterior cruciate ligaments from 66 rabbits underwent the freeze-thaw treatment, and animals were then divided into 3 groups. Group I served as a freeze-thaw but otherwise untreated control. In group II, 0.2 mL phosphate-buffered saline alone was applied. In group III, 30 mu g vascular endothelial growth factor was applied. The groups were compared on the basis of histologic revascularization examinations using the Chalkley score, an indicator of the microvessel density, and mechanical evaluations, which included the anterior-posterior translation of the tibia relative to the femur during +/- 10 N of anterior-posterior load and the mechanical properties of the anteromedial bundle of the anterior cruciate ligament.Results: Group III's Chalkley score was significantly greater than that of groups I and II. The tensile strength and the tangent modulus of anterior cruciate ligaments in groups I, II, and III were significantly lower than those of a normal anterior cruciate ligament, although there were no significant differences among groups I, II, and III.Conclusion: Vascular endothelial growth factor, as administered in this study, significantly promoted angiogenesis in the devitalized anterior cruciate ligament with in situ freeze-thaw treatment, but it did not affect the mechanical properties of the in situ frozen-thawed anterior cruciate ligament in the rabbit model.Clinical Relevance: An application of the recombinant anterior cruciate ligament is a potential future strategy to enhance revascularization of the autograft in anterior cruciate ligament reconstruction.