A COMPARISON OF SPLEEN AND LYMPH-NODE CELLS AS FUSION PARTNERS FOR THE RAISING OF MONOCLONAL-ANTIBODIES AFTER DIFFERENT ROUTES OF IMMUNIZATION
A COMPARISON OF SPLEEN AND LYMPH-NODE CELLS AS FUSION PARTNERS FOR THE RAISING OF MONOCLONAL-ANTIBODIES AFTER DIFFERENT ROUTES OF IMMUNIZATION
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DOI：
10.1016/0022-1759(87)90271-7
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发表时间：
1987-12-24
影响因子：
2.2
通讯作者：
MOORE, K
中科院分区：
文献类型：
--
作者：
MIRZA, IH;WILKIN, TJ;MOORE, K
通过BALB/c小鼠后足垫给予抗原（人胰岛素），随后融合腘淋巴结和腹股沟淋巴结淋巴细胞，诱导分泌特异性抗体的杂交瘤（100%）的频率高于皮内免疫和淋巴结细胞融合（53%）或常规皮下免疫和腹膜内加强免疫，随后脾淋巴细胞融合（85%）。发现血清抗体滴度的等级顺序与融合效率的顺序相关。淋巴结细胞融合也产生了更大的抗体特异性谱。使用牛肠碱性磷酸酶也观察到融合效率的这种差异。
Administration of antigen (human insulin) via the rear footpads of BALB/c mice with subsequent fusion of popliteal and inguinal lymph node lymphocytes induced a higher frequency of hybridomas (100%) secreting specific antibody than either intradermal immunisation and lymph node cell fusion (53%) or conventional subcutaneous immunisation and intraperitoneal boost followed by splenic lymphocyte fusion (85%). The rank order of serum antibody titres was found to correlate with the order of fusion efficiencies. Lymph node cell fusions also produced a greater spectrum of antibody specificities. Such differences in fusion efficiencies were also observed using bovine intestinal alkaline phosphatase.