Expression analysis of transporter genes for screening candidate monolignol transporters using Arabidopsis thaliana cell suspensions during tracheary element differentiation
Expression analysis of transporter genes for screening candidate monolignol transporters using Arabidopsis thaliana cell suspensions during tracheary element differentiation
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DOI：
10.1007/s10265-017-0979-4
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发表时间：
2018-03-01
影响因子：
2.8
通讯作者：
Tsutsumi, Yuji
中科院分区：
文献类型：
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作者：
Takeuchi, Manami;Kegasa, Takahiro;Tsutsumi, Yuji
尽管单体从胞质到质外体的运输是木质化的一个重要步骤，但其机制仍不清楚。最近，ATP结合盒（ABC）转运蛋白被认为参与了单细胞转运。然而，没有可靠的线索，主要的木质素单体松柏醇和synapyl醇的转运蛋白。本研究对拟南芥培养细胞导管分子分化过程中的木质化进程进行了监测。通过实时PCR分析以2天间隔收获的分化培养细胞样品中所选转运蛋白基因以及作为参考的木质化和细胞壁形成相关基因的表达，并对数据进行统计学处理。细胞壁形成转录因子MYB 46、细胞程序性死亡相关基因XCP 1和木质素聚合过氧化物酶AtPrx 25被归为同一簇。此外，最接近上述簇的簇包含木质素合成转录因子MYB 58和拟南芥ABC转运蛋白ABCG 11、ABCG 22、ABCG 36和ABCG 29。这一结果表明，这四个ABC转运蛋白可能参与木质化。在表达分析中，出乎意料地，木质化相关基因CAD 5和C4 H不包括在与MYB 58和AtPrx 25相同的簇中。表达数据还表明，培养物中的导管元件的木质化，其中木质化比率最终达到约40%，在细胞死亡后继续，因为在程序性细胞死亡相关基因开始表达后，木质化积极进行。
The mechanism of monolignol transportation from the cytosol to the apoplast is still unclear despite being an essential step of lignification. Recently, ATP-binding cassette (ABC) transporters were suggested to be involved in monolignol transport. However, there are no reliable clues to the transporters of the major lignin monomers coniferyl and synapyl alcohol. In this study, the lignification progress of Arabidopsis cultured cells during tracheary element differentiation was monitored. The expression of selected transporter genes, as well as lignification and cell-wall formation related genes as references, in differentiating cultured cell samples harvested at 2-day intervals was analyzed by real-time PCR and the data were statistically processed. The cell wall formation transcription factor MYB46, programmed-cell death related gene XCP1 and lignin polymerization peroxidase AtPrx25 were classified into the same cluster. Furthermore, the cluster closest to the abovementioned cluster contained the lignin synthesis transcription factor MYB58 and the Arabidopsis ABC transporters ABCG11, ABCG22, ABCG36 and ABCG29. This result suggested that these four ABC transporters may be involved in lignification. In the expression analysis, unexpectedly, the lignification-related genes CAD5 and C4H were not included in the same cluster as MYB58 and AtPrx25. The expression data also suggested that the lignification of tracheary elements in the culture, where lignification ratio finally reached to around 40%, continued after cell death because lignification actively progressed after programmed cell death-related gene started to be expressed.