Expression of CD44s and CD44v6 in transitional cell carcinomas of the urinary bladder:: comparison with tumour grade, proliferative activity and p53 immunoreactivity of tumour cells
Expression of CD44s and CD44v6 in transitional cell carcinomas of the urinary bladder:: comparison with tumour grade, proliferative activity and p53 immunoreactivity of tumour cells
复制标题
DOI：
10.1111/j.1600-0643.2007.00602.x
复制
发表时间：
2007-11-01
期刊：
影响因子：
2.8
通讯作者：
Mandys, Vaclav
中科院分区：
文献类型：
--
作者：
Kuncova, Jitka;Urban, Michael;Mandys, Vaclav
CD44 糖蛋白的改变已被证明在包括尿路上皮癌在内的各种恶性肿瘤的进展中发挥重要作用。我们研究了膀胱移行细胞癌 (TCC) 中 CD44s 和 CD44v6 的表达模式与肿瘤分级、增殖活性和 p53 免疫反应性的关系。对 122 个 TCC 样本进行免疫组织化学检测。我们发现 CD44s 和 CD44v6 表达与肿瘤分级之间存在密切关系。 CD44s 和 CD44v6 的阳性染色向管腔表面延伸是分化癌（I 级和 2 级）的主要特征，表明癌细胞的成熟紊乱与其肿瘤转化有关。 CD44s 和 CD44v6 的异质表达在低分化肿瘤 (G3-4) 中占主导地位。然而，高级别肿瘤内的鳞状分化区域对 CD44 和 CD44v6 均表现出强烈的免疫反应性。增殖活性和p53过表达随着肿瘤的去分化而增加。本研究的结果讨论了 TCC 中 CD44 表达的重要性，以及对先前关于 CD44 表达与尿路上皮细胞生物学行为之间关系的研究中报告的有争议结果的解释。
Alterations of CD44 glycoproteins have been shown to play an important role in progression of various malignancies, including urothelial cancer. We investigated expression patterns of CD44s and CD44v6 in transitional cell carcinoma (TCC) of the urinary bladder in relation to tumour grade, proliferative activity, and immunoreactivity for p53. The selected markers were detected immunohistochemically in 122 samples of TCC. We found a close relationship between CD44s and CD44v6 expression and tumour grade. The extension of positive staining for CD44s and CD44v6 towards the luminal surface was a predominant feature of differentiated carcinomas (grades I and 2), suggesting deranged maturation of cancer cells related to their neoplastic transformation. Heterogeneous expression of CD44s and CD44v6 predominated in poorly differentiated tumours (G3-4). However, areas of squamous differentiation within the high-grade tumours displayed strong immunoreactivity for both CD44s and CD44v6. The proliferative activity and p53 overexpression increased with the dedifferentiation of the tumour. The results of this study are discussed in relation to the significance of CD44 expression in TCC and to the explanation for controversial results reported in previous studies on the relationship between CD44 expression and the biological behaviour of urothelial cells.