Stimulation of growth of keratinocytes by basic fibroblast growth factor.
Stimulation of growth of keratinocytes by basic fibroblast growth factor.
复制标题
碱性成纤维细胞生长因子刺激角质形成细胞的生长。
DOI：
10.1111/1523-1747.ep12560956
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
PayneJr,RE
中科院分区：
文献类型：
--
作者：
O'Keefe,EJ;Chiu,ML;PayneJr,RE
最近发现，许多在不同类型细胞中具有活性的肝素结合生长因子都属于成纤维细胞生长因子家族。因为这些因子在某些类型的上皮细胞中是活跃的，所以我们测试了来自牛脑的碱性成纤维细胞生长因子（bFGF）在人角质形成细胞培养物中的活性。bFGF在这些培养物中刺激胸苷掺入和细胞增殖，半数最大活性约为60 pg/ml（4× 10- 12 M）。胸苷掺入的刺激与增加核标记后22小时，在碱性成纤维细胞生长因子的存在下，在胸苷掺入试验中使用的相同条件下。bFGF在刺激角质形成细胞生长方面几乎与表皮生长因子（EGF）一样有效，而在刺激胸苷掺入或细胞增殖方面与EGF相比效果明显较差。研究结果表明，bFGF是一种有效的角质形成细胞的生长因子。
Numerous heparin-binding growth factors active in different types of cells have recently been shown to belong to the family of fibroblast growth factors. Because these factors are active in some types of epithelial cells, we tested the activity of basic fibroblast growth factor (bFGF) from bovine brain in human keratinocyte cultures. bFGF stimulated thymidine incorporation and cellular proliferation in these cultures with half-maximal activity at approximately 60 pg/ml (4× 10-12M). Stimulation of thymidine incorporation was associated with increased nuclear labeling after 22 h in the presence of bFGF under the same conditions used in the thymidine incorporation assay. bFGF was nearly as effective as epidermal growth factor (EGF) in stimulating keratinocyte growth and substantially less effective than crude placental extract, and was not additive with EGF in stimulating thymidine incorporation or proliferation of cells. The findings indicate that bFGF is a potent growth factor for keratinocytes.
牛脑中多种形式的前列腺素（前列腺上皮细胞生长因子）的表征。
DOI：
--
发表时间：
1986
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
J. Crabb;L. Armes;Charles M. Johnson;W. Mckeehan
通讯作者：
W. Mckeehan
肝素对人酸性和碱性FGF刺激非血管细胞的影响。
DOI：
--
发表时间：
1986
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
S. Uhlrich;O. Lagente;M. Lenfant;Y. Courtois
通讯作者：
Y. Courtois
影响因子：
2.9
作者：
OLWIN, BB;HAUSCHKA, SD
通讯作者：
HAUSCHKA, SD