Practical procedures for the integrated diagnosis of astrocytic and oligodendroglial tumors
Practical procedures for the integrated diagnosis of astrocytic and oligodendroglial tumors
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DOI：
10.1007/s10014-019-00337-y
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发表时间：
2019-04-01
影响因子：
3.3
通讯作者：
Nishihara, Hiroshi
中科院分区：
文献类型：
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作者：
Sonoda, Yukihiko;Yokoo, Hideaki;Nishihara, Hiroshi
2016年世界卫生组织中枢神经系统肿瘤分类（2016 WHO CNS）的发布代表了脑肿瘤分类的重大变化。然而，由于资金或技术问题，日本许多病理学家无法根据2016年WHO CNS诊断星形细胞或少突胶质细胞肿瘤。因此，日本脑肿瘤病理学会成立了分子诊断委员会，以促进日本星形细胞肿瘤和少突胶质细胞肿瘤的综合诊断。我们创建了三个诊断级别：1 级定义为使用苏木精和伊红染色以及基于常规细胞谱系的免疫染色的简单组织病理学诊断。 2级被定义为使用R132H突变特异性IDH1、ATRX和/或p53抗体进行免疫组织化学检查的免疫组织化学诊断。 3级定义为分子诊断，例如基于1p/19q状态或IDH1和IDH2基因突变状态的诊断。原则上，星形细胞肿瘤和少突胶质细胞肿瘤应根据2016年WHO CNS和/或cIMPACT-NOW标准进行诊断；然而，通过我们的诊断流程图获得的结果可以添加到括号中的组织学诊断中。这种分类系统对于资源有限的病理学家来说将是有帮助的。
The publication of the 2016 World Health Organization Classification of Tumors of the Central Nervous System (2016 WHO CNS) represented a major change in the classification of brain tumors. However, many pathologists in Japan cannot diagnose astrocytic or oligodendroglial tumors according to the 2016 WHO CNS due to financial or technical problems. Therefore, the Japan Society of Brain Tumor Pathology established a committee for molecular diagnosis to facilitate the integrated diagnosis of astrocytic and oligodendroglial tumors in Japan. We created three levels of diagnoses: Level 1 was defined as simple histopathological diagnosis using hematoxylin and eosin staining and routine cell lineage-based immunostaining. Level 2 was defined as immunohistochemical diagnosis using immunohistochemical examinations using R132H mutation-specific IDH1, ATRX, and/or p53 antibodies. Level 3 was defined as molecular diagnosis, such as diagnosis based on 1p/19q status or the mutation status of the IDH1 and IDH2 genes. In principle, astrocytic and oligodendroglial tumors should be diagnosed based on the 2016 WHO CNS and/or cIMPACT-NOW criteria; however, the findings obtained through our diagnostic flowchart can be added to the histological diagnosis in parentheses. This classification system would be helpful for pathologists with limited resources.